Purification and Characterization of an Extracellular β-Agarase from Bacillus sp.MK03
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概要
- 論文の詳細を見る
A new β-agarase was purified from an agarolytic bacterium, Bacillus sp.MK03. The enzyme was purified 129-fold from the culture supernatant by ammonium sulfate precipitation, anion exchange and gel filtration column chromatographic methods. The purified enzyme appeared as a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Estimation of the molecular mass by SDS-PAGE and gel filtration gave values of 92 kDa and 113 kDa, respectively. The N-terminal amino acid sequence of the enzyme showed no homology to those of other known agarases. The optimum pH and temperature for this enzyme were 7.6 and 40℃, respectively. The predominant hydrolysis product of agarose by this enzyme was neoagarotetraose, indicating the cleavage of β-1,4 linkage. This enzyme could hydrolyze neoagarohexaose to produce neoagarotetraose and neoagarobiose ; it could not hydrolyze these products. The enzyme digested agarose by endo-type hydrolysis.
- 公益社団法人日本生物工学会の論文
- 2003-04-25
著者
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Kawai K
Faculty Of Applied Biological Sciences Gifu University
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Suzuki T
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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SUZUKI Tohru
Molecular Genetics Reseaech Center
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Kawai Keiichi
Faculty Of Applied Biological Sciences Gifu University
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Kawai K
Fac. Appl. Biol. Sci. Gifu Univ.
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Kawai Keiichi
School Of Health Sciences Faculty Of Medicine Kanazawa University
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Suzuki H
Nagoya Univ. Nagoya Jpn
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Suzuki Hisashi
Laboratory Of Molecular Biotechnology Graduate School Of Biological And Agricultural Sciences Nagoya
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SUZUKI HISASHI
The United Graduate School of Agricultural Science, Gifu University
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SAWAI YOSHINORI
Research Institute of Industrial Products,Gifu Prefectural Government
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Sawai Y
Research Institute Of Industrial Products Gifu Prefectural Government
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SUZUKI Tohru
Faculty of Applied Biological Sciences, Gifu University
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Kawai Keiichi
Fac. Appl. Biol. Sci. Gifu Univ.
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Kamimura Kiyohisa
Department Of Radiology Fujimoto Hayasuzu Hospital
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