Cloning and Expression of a 58-kDa Xylanase VI Gene (xynD) of Aeromonas caviae ME-1 in Escherichia coli Which is not Categorized as a Family F or Family G Xylanase

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概要

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• An endo-xylanase gene (xynD) encoding xylanase VI(58 kDa) of Aeromonas caviae ME-1 was cloned and expressed in Escherichia coli. The xynD gene encodes a 564 amino acid precurosr (62,083 Da) and 531 amino acid mature enzyme (58,469 Da). the enzyme consists of two domains bridged by a glycine-rich linker. The N-terminal catalytic domain (392 amino acids) sequence is homologous neither to family F nor faimily G (families 10,11) enzymes, into which almost all known xylanases are categorized. However, it has 41% identity with XynA of Erwinia chrysanthemi. The C-terminal domain (125 amino acids) shows homology to conserved domains in xylanase (XYLB), arabino-furanosidase (XYLC), and acetylxylan-esterase (XYLD) of Pseudomonas fluorescens subsp. cellulosa, but its function is unknown. The E. coli transformant produced 58-, 48-, 45-, and 43-kDa active xhylanases as the result of C-terminal proteolytic truncation.

• 社団法人日本生物工学会の論文
• 1997-07-25

著者

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• HATSU Masahiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• TAKAMIZAWA Kazuhiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Takamizawa Kazuhiro

  Faculty of Applied Biological Sciences, Gifu University

• IBATA Keiji

  Department of Biotechnology, Faculty of Agriculture, Gifu University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Ibata Keiji

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Takamizawa K

  Faculty Of Applied Biological Sciences Gifu University

• Hatsu M

  Gifu Univ. Gifu Jpn

• Hatsu Masahiro

  Department Of Bioprocessing Faculty Of Agriculture Gifu University

• Takamizawa Kazuhiro

  Department Of Agriculture Gifu University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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