Purification, Characterization and Structure Analysis of NADPH-Dependent D-Xylose Reductases from Candida tropicalis

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概要

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• NADPH-dependent D-xylose reductases (XRs) from Candida tropicalis IFO 0618 were purified and characterized. Mono Q HPLC revealed three XR isomers. The K_m values of XR1,XR2,and XR3 for D-xylose were 37,30 and 34 mM, and for NADPH 14,18 and 9 μM, respectively. NADH did not act as a cofactor. The specificties of the three XRs for several aldoses were essentially the same. Gel filtration and cross-linking analysis showed that both XR1 and XR2 were dimers composed of identical subunits. The pI values of XR1 and XR2 were estimated to be 4.15 and 4.10,respectively. Comparison of the peptide maps of XR1 and XR2 showed that the molecular weights of 8 fragments of lysylendopeptidase-digested XR1 and XR2 were essentially the same as each other. The amino acid composition of each XR was also very similar. The molecular weights of XR1 and XR2 by mass spectra analysis were 36,497.91and 36,539.68,respectively. The amino acid sequences of two XR1 peptide fragments (Nos. 4 and 5) were highly homologous with those of Pichia stipitis XR and mammalian aldose reductases.

• 社団法人日本生物工学会の論文
• 1995-03-25

著者

• Yokoyama S

  Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Yokoyama S

  Takara Shuzo Co. Ltd. Shiga Jpn

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• TAKAMIZAWA Kazuhiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Horitsu Hiroyuki

  Department ofBiotechnology, Division of Bioresources Utilization, Faculty ofAgriculture,Gifu Univ.

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Takamizawa Kazuhiro

  Faculty of Applied Biological Sciences, Gifu University

• YOKOYAMA SHIN-ICHIRO

  United Graduate School of Agricultural Science

• Horitsu Hiroyuki

  Department Of Biotechnology Faculty Of Agriculture:(present Address)facculty Of Home Economics Depar

• Horitsu Hiroyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Takamizawa K

  Faculty Of Applied Biological Sciences Gifu University

• Horitsu H

  Department Of Biotechnology Faculty Of Agriculture:(present Address)facculty Of Home Economics Depar

• Takamizawa Kazuhiro

  Department Of Agriculture Gifu University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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