New Rapid Enzyme-Linked Immunosorbent Assay to Detect Antibodies against Bacterial Surface Antigens Using Filtration Plates
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概要
- 論文の詳細を見る
An easy and rapid ELISA system, Filtration ELISA, to detect antibodies against bacterial cell surface antigens was developed using a 96-well filtration plate fitted with a 0.22μm membrane (MultiScreen^<[○!R]>-GV, Millipore). Bacterial whole cells were used as antigens without fixing the cells with formalin etc. The whole cell antigens were washed by vacuum filtration through a filter and resuspended in washing buffer. Assay reactions could be done in the wells without losing the solution. The technique was established using antisera of mice immunized with Escherichia coil, and then evaluated by assaying antibodies to Shiga-toxin producing E. coli O157:H7 (STEC), Staphylococcus aureus and Lactobacillus acidophilus in fecal extracts of 157 children who had eaten school lunches contaminated with STEC in comparison with 25 age-matched control children. The lunch group showed significantly higher IgA antibody titers against STEC than the control group (p<0.0005), but not against L. acidphilus. The results indicate that Filtration ELISA is a quantitative and specific technique for measuring antibodies against antigens on the surface of bacteria without extracting antigens from the bacteria. This technique is widely applicable to the assay of antibodies in various samples including serum and fecal extract against various kinds of bacteria.
- 公益社団法人日本薬学会の論文
- 2002-08-01
著者
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Yokoyama S
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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Yokoyama S
Takara Shuzo Co. Ltd. Shiga Jpn
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Yokoyama Shin-ichiro
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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MORI Hiroshi
Laboratory of Microbiology, Gifu Pharmaceutical University
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NAGANO Keiji
Laboratory of Microbiology, Department of Public Health Pharmacy, Gifu Pharmaceutical University
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ITOH Saori
Laboratory of Microbiology, Department of Public Health Pharmacy Gifu Pharmaceutical University
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KARIYA Masako
Laboratory of Microbiology, Department of Public Health Pharmacy Gifu Pharmaceutical University
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FUKAO Toshio
Gifu Municipal Institute of Public Health
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YAMAZAKI Yoshihisa
Department of Pediatrics, Prefectural Gifu Hospital
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Nagano K
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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Fukao T
Gifu Municipal Institute Of Public Health
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Kariya Masako
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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Nagano Keiji
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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Yamazaki Yoshihisa
Department Of Pediatrics Prefectural Gifu Hospital
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Yamazaki Yoshihisa
Department Of Pediatrics Gifu Prefectural Gifu Hospital
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Mori Hiroshi
Laboratory Of Microbiology Department Of Public Health Pharmacy Gifu Pharmaceutical University
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