Processing of XynE (110-kDa) of Aeromonas caviae ME-1 to 72-kDa Xylanase in Eseherichia coli Transformant
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概要
- 論文の詳細を見る
An extracellular 72-kDa xylanase from an Eseherichia coli transformant that harbored pXED30 carrying xynE of Aeromonas caviae ME-1 was purified by extraction following SDS-PAGE to electrophoretic homogeneity. The analysis of N-terminal 10 amino acid residues (Gly-Ala-Arg-Ala-Gln-Ala-Ala-Ala-Asp-Val) revealed a 72-kDa product derived by the cleavage of Gly_<26>-Gly_<27> at the N-terminal region of 110 kDa XynE. The 72-kDa xylanase from A. caviae ME-1 was found to have the same sequence as that of the N-terminal 10 amino acid residues. When OmpT-deficient E. coli mutants were used as hosts, the 72-kDa xylanase was detected in cell-free extracts, but not in the culture supernatant, suggesting that OmpT is not involved in the cleavage at the C-terminal region, but is involved in the secretion of 72-kDa xylanase to the culture medium.
- 社団法人日本生物工学会の論文
- 2003-10-25
著者
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Kawai K
Faculty Of Applied Biological Sciences Gifu University
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Kawai Keiichi
Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ
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Suzuki Tohru
Life Science Research Center, Gifu University
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Suzuki T
Ihara & Company Ltd.
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Suzuki Tohru
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Liu Chen
United Graduate School Of Agricultural Science Gifu University
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Kawai K
Fac. Appl. Biol. Sci. Gifu Univ.
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Kawai Keiichi
School Of Health Sciences Faculty Of Medicine Kanazawa University
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Hirata Satoru
Department Of Biotechnology Faculty Of Agriculture
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Kawai Keiichi
Fac. Appl. Biol. Sci. Gifu Univ.
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Kawai Keiichi
Department Of Bioresources Utilization Faculty Of Agriculture Gifu University
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Suzuki Tohru
Life Science Research Center Gifu University
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Kamimura Kiyohisa
Department Of Radiology Fujimoto Hayasuzu Hospital
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KAWAI Keiichi
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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