Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas ambigua G-1
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概要
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When hexavalent chromium (Cr6+) tolerant Pseudomonas ambigua G-1 was cultivated in nutrient broth containing 150ppm Cr6+, the Cr6+ content of the broth rapidly decreased. The Cr6+ reducing enzyme found in a cell-free extract of P. ambigua G-1 required NADH but not NADPH as a hydrogen donor for the reduction of Cr6+. The specific activities of cell-free extracts of several Cr6+ sensitive mutants derived from P. ambigua G-1 showed decreases to one fourth to one tenth of that of P. ambigua G-1. Glucose protected the Cr6+ reducing enzyme against inactivation on dialysis.
- 社団法人 日本農芸化学会の論文
著者
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Horitsu Hiroyuki
Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University
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Kawai Keiichi
Department Of Bioresources Utilization Faculty Of Agriculture Gifu University
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FUTO Satoshi
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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MIYAZAWA Yoshimi
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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OGAI Shusuke
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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KAWAI Keiichi
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
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