XynX, a Possible Exo-xylanase of Aeromonas caviae ME-1 that Produces Exclusively Xylobiose and Xylotetraose from Xylan

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概要

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• A gene, xynX, encoding a novel xylanase, was cloned from Aeromonas caviae ME-1. This gene encoded an enzyme that was constituted of 334 amino acid residues (38,580 Da) and was similar in sequence to Family 10 (Family F) β-1,4 endo-xylanases. XynX produced produced only xylobiose and xylotetraose from birch wood xylan, and xylotriose, xylopentaose, and higher oligosaccharides were not detected in the TLC analysis. We designated it as X2/X4-forming xylanase. This enzyme does not have transglycosylation activity. These data suggested that this enzyme is a possible exo-xylanase. According to homology modeling, the enzyme has a ring-shaped (α/β)_8 barrel (TIM barrel) structure, typical of Family 10 endo-xylanases, with the extraordinary feature of a longer bottom-loop structure.

• 社団法人日本農芸化学会の論文
• 1999-08-23

著者

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• USUI KENGO

  Department of Biotechnology, United Graduate School Agricultural Science, Department of Biotechnolog

• IBATA Keiji

  Department of Biotechnology, Faculty of Agriculture, Gifu University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Suzuki T

  Ihara & Company Ltd.

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Usui Kengo

  Department Of Biotechnology United Graduate School Agricultural Science Department Of Biotechnology

• Ibata Keiji

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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