Cloning and Expression of a NAD^+-Dependent Xylitol Dehydrogenase Gene (xdhA) of Aspergillus oryzae(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
XdhA, which encodes a xylitol dehydrogenase gene, was cloned from Aspergillus oryzae genomic DNA. It consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 Da). It is similar to the known NAD^+-dependent xylitol dehydrogenase (EC 1.1.1.9). The gene was expressed in Escherichia coli BL21-AI using a T7 promoter. The cell-free extract of the transformant showed a 36.5 kDa band upon SDS-PAGE and NAD^+- dependent xylitol dehydrogenase activity.
- 社団法人日本生物工学会の論文
- 2004-06-25
著者
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鈴木 徹
岐阜大院・連農・生資科
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KAWAI Keiichi
Faculty of Health Science, School of Medicine, Kanazawa University
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SUZUKI Tohru
United Graduate School of Agricultural Science, Gifu University
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Suzuki T
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Tran Lien
Institute Of Biological Food Technology Hanoi University Of Technology
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Suzuki Tohru
Life Science Research Center, Gifu University
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Kitamoto Noriyuki
Food Research Center, Aichi Industrial Technology Institute
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Takamizawa Kazuhiro
Faculty of Applied Biological Sciences, Gifu University
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Saito T
Kanagawa Univ. Kanagawa Jpn
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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Suzuki T
Ihara & Company Ltd.
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Suzuki Tohru
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Kawai Keiichi
Faculty Of Applied Biological Sciences Gifu University
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Takamizawa K
Faculty Of Applied Biological Sciences Gifu University
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TRAN LIEN
United Graduate School of Agricultural Science, Gifu University
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Kitamoto N
Food Research Center Aichi Industrial Technology Institute
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Kitamoto Noriyuki
Food Research Center Aichi Industrial Technology Institute
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SUZUKI Tohru
Faculty of Applied Biological Sciences, Gifu University
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Suzuki Tohru
The United Graduate School Of Agricultural Science Gifu University
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Suzuki Tohru
Molecular Genetics Research Center Gifu University
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Suzuki Tohru
Life Science Research Center Gifu University
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Suzuki Tohru
United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.
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Kitamoto Noriyuki
Food Res. Center Aichi Ind. Tech. Inst.
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SUZUKI Tohru
The United Graduate School of Agricultural Science, Gifu University
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Takamizawa Kazuhiro
Faculty of Applied Biological Science, Gifu University
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