Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression.
著者
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鈴木 徹
岐阜大院・連農・生資科
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SUZUKI Tohru
United Graduate School of Agricultural Science, Gifu University
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Suzuki T
Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University
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Suzuki Tohru
Life Science Research Center, Gifu University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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SUZUKI Tohru
Faculty of Applied Biological Sciences, Gifu University
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Suzuki Tohru
The United Graduate School Of Agricultural Science Gifu University
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Suzuki Tohru
Molecular Genetics Research Center Gifu University
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Suzuki Tohru
United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.
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He Jianlong
The United Graduate School Of Agricultural Science Gifu University
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SUZUKI Tohru
The United Graduate School of Agricultural Science, Gifu University
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Sakaguchi Kouta
The United Graduate School of Agricultural Science, Gifu University
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