Time-Dependent Variation of the Surface Sturcture of Bioceramics in Tissue Culture Medium and the Effect on Adhesiveness of Cells

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概要

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• Biocompatible ceramics made of β-tricalcium phosphate (TCP) were found to be actively changing their surface characteristics in tissue culture medium. The time-dependent variation of the zeta potential of TCP ceramics immersed in distilled water and in culture medium with and without addition of fetal bovine serum showed that the surface was unstable with significant changes in the charge being measured. Dry TCP had a zeta potential of -9.3 mV, which shifted to -1.8 mV after soaking in water and to -27.6 mV in culture medium with serum. To clarify the effect of the time-dependent variation of the surface structure on growth and adhesion of anchorage-dependent animal cells, the zeta potential of ceramics in dry state was regulated from -11.5 mV to +27.2 mV by means of silane coupling modification. After soaking in distilled water for six days, zeta potential of the modified TCPs shifted to between +7.5 mV and -7.6 mV, while they were betweeen -9.9 mV and -23.7 mV in culture medium with serum. Concentrations of calcium and phosphate dissolved in distilled water and in culture medium showed the solubility of the ceramics was considerably high and depended on the pH of the surface layer. The suitable surface property for adhesion of L-929 cells was obtained by the most stable ceramics in the culture condition. In conclusion, the solubility of the thin surface layer of the carrier was considered to be the dominant factor in decreasing the adhesiveness of cells on TCP ceramics.

• 社団法人日本生物工学会の論文
• 1996-03-25

著者

• NAGATA Fukue

  National Institute of Advanced Industrial Science and Technology

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• KAWAMOTO YURI

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• NISHIZAWA KAORI

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• NAGATA FUKUE

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• YOKOGAWA YOSHIYUKI

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• KAWAMOTO YUKARI

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• KAMEYAMA TETSUYA

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• SUZUKI TAKAHIRO

  Bioceramics Laboratory, National Industrial Research Institute of Nagoya

• Kawamoto Y

  Bioceramics Laboratory National Industrial Research Institute Of Nagoya

• Kameyama T

  National Industrial Res. Inst. Nagoya Nagoya‐shi

• Kawamoto Yuri

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Nishizawa K

  National Industrial Research Institute Of Nagoya

• Yokogawa Yoshiyuki

  National Institute Of Advanced Industrial Science And Technology (aist)

• Kameyama Tetsuya

  Bioceramics Laboratory National Industrial Research Institute Of Nagoya

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