Effects of the Surface Wettability and Zeta Potential of Bioceramics on the Adhesiveness of Anchorage-Dependent Animal Cells

スポンサーリンク

概要

• 論文の詳細を見る

• Using calcium phosphate ceramics that have high biocompatibility with the living body, the effects of the surface characteristics of the bioceramics on cell adhesiveness were investigated. In the case of carriers with contact angles from 35° to 60°, the cell adhesiveness increased according to the increase in the wettability. Measurement of the zeta potentials of HAP-TCP sinters showed that these bioceramics had negative potentials from -2 mV to -6 mV. Electrochemical analysis suggested that the initial cell anchoring ratio (R_<ia>) and adhesive strength (F_<a.enz>) were affected by the surface ionic condition of the ceramic material. To clarify the effects of the surface potential of the ceramics on cell adhesiveness, the ceramic surface was modifed chemically by means of various silane coupling reagents. The surface potential was regulated from -20 mV to +24 mV.Using these ceramics, the affinity and adhesiveness of the cells to the ceramics were found to be dominantly regulated by the surface potential. A negative potential was effective in increasing the adhesiveness, even though living cells have negative charges.

• 社団法人日本生物工学会の論文
• 1993-06-25

著者

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• KAWAMOTO YURI

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• NISHIZAWA KAORI

  Government Industrial Research Institute, Nagoya

• TORIYAMA MOTOHIRO

  Government Industrial Research Institute, Nagoya

• SUZUKI TAKAHIRO

  Government Industrial Research Institute, Nagoya

• KAWAMOTO YUKARI

  Government Industrial Research Institute, Nagoya

• YOKOGAWA YOSHIYUKI

  Government Industrial Research Institute, Nagoya

• NAGAE HAJIME

  Government Industrial Research Institute, Nagoya

• Nagashima H

  Sci. Univ. Tokyo Chiba Jpn

• Kawamoto Y

  Bioceramics Laboratory National Industrial Research Institute Of Nagoya

• Toriyama M

  Ceramics Research Institute National Institute Of Advanced Industrial Science And Technology

• Toriyama Motohiro

  Government Industrial Research Institute Nagoya

• Kawamoto Yuri

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Nishizawa K

  National Industrial Research Institute Of Nagoya

• Yokogawa Yoshiyuki

  National Institute Of Advanced Industrial Science And Technology (aist)

関連論文

• Primary Structure and Catalytic Properties of a Cold-active Esterase from a Psychrotroph, Acinetobacter sp. Strain No.6. Isolated from Siberian Soil(Biochemistry & Molecular Biology)
• Cold-Active Lipolytic Activity of Psychrotrophic Acinetobacter sp. Strain No.6
• Expression of a Thermostable Cellulase Gene from a Thermophilic Anaerobe in Saccharomyces cerevisiae
• Purification and Characterization of D-Xylose Isomerase from Bifidobacterium adolescentis
• Feasibility of Induction Chemotherapy Using Bronchial Arterial Infusion for Locally Advanced Non-Small Cell Lung Cancer : A Pilot Study
• Successful Induction of Tumor-specific Cytotoxic T Lymphocytes from Patients with Non-small Cell Lung Cancer Using CD8O-transfected Autologous Tumor Cells
• Interleukin-12 enhances the antitumor activity of cytotoxic T lymphocytes immunodeficient mice
• Effects of Interleukin-12 on the Induction of Cytotoxic T Lymphocytes from the Regional Lymph Node Lymphocytes of Patients with Lung Adenocarcinoma
• Optimization of Kojic Acid Production Rate Using the Box-Wilson Method
• Xylanase I of Aeromonas caviae ME-1 Isolated from the Intestine of a Herbivorous Insect (Samia cynthia pryeri)
• Development of a Culture Carrier for Anchorage-Dependent Animal Cells Using Calcium Phosphate Ceramic Sinters
• Production of Recombinant Human Monoclonal Antibody Using ras-Amplified BHK-21 Cells in a Protein-free Medium
• Cloning and Expression of NAD^+-Dependent L-Arabinitol 4-Dehydrogenase Gene (ladA) of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• 2F11-4 Xylitol dehydrogense genes of Aspergillus oryzae
• A Cytoplasmic Xylanase (XynX) of Aeromonas caviae ME-1 Is Released from the Cytoplasm to the Periplasm by Osmotic Downshock
• Construction of deletion mutants of Shiga(-like) toxin genes(stx-1 and/or stx-2) on enterohemorrhagic Escherichia coli(O157 : H7).
• Nucleotide sequence analysis of Shiga(-like) toxins from an exterohemorrhagic Escherichia coli isolated from the gifu, Japan, outbreak.
• Cloning, Expression, and Characterization of a Family 52 β-Xylosidase Gene (xysB) of a Multiple-xylanase-producing Bacterium, Aeromonas caviae ME-1
• XynX, a Possible Exo-xylanase of Aeromonas caviae ME-1 that Produces Exclusively Xylobiose and Xylotetraose from Xylan
• Expression of xyrA Gene Encoding for D-Xylose Reductase of Candida tropicalis and Production of Xylitol in Escherichia coli
• Cloning and Expression of a 58-kDa Xylanase VI Gene (xynD) of Aeromonas caviae ME-1 in Escherichia coli Which is not Categorized as a Family F or Family G Xylanase
• Cloning and Expression of Xylanase I Gene (xynA) of Aeromonas caviae ME-1 in Escherichia coli
• Production of Xylitol from D-Xylose by Candida tropicalis : the Effect of D-Glucose Feeding
• Cloning and Sequencing of Two D-Xylose Reductase Genes (xyrA and xyrB) from Candida tropicalis
• Production of Worcestershire Sauce by a Trickle Bed Bioreactor Using Comb-Shaped Ceramics Carriers
• Purification, Characterization and Structure Analysis of NADPH-Dependent D-Xylose Reductases from Candida tropicalis
• Cadmium Resistance Acquirement by IS1 Transposition into Escherichia coli C600
• A Study of Yeast Adsorption on Ceramic Beads by Zeta Potential
• Orientation of Hydroxyapatite C-Axis under High Magnetic Field with Mold Rotation and Subsequent Sintering Process
• Formation of c-axis Aligned Polycrystal Hydroxyapatite Using High Magnetic Field with Mechanical Sample Rotation
• A Mini-Scale Mass Production and Separation System for Secretory Heterologous Proteins by Perfusion Culture of Recombinant Pichia pastoris Using a Shaken Ceramic Membrane Flask
• High-Level Expression of the Methanol-Inducible β-Galactosidase Gene by Perfusion Culture of Recombinant Pichia pastoris Using a Shaken Ceramic Membrane Flask
• Continuous Production and Separation of Ethanol without Effluence of Wastewater Using a Distiller Integrated SCM-Reactor System
• Ethanol Production in an Immobilized Cell Reactor Couped with the Recycling of Effluent from the Bottom of a Distillation Column
• Production of a Recombinant Human Monoclonal Antibody Using a Novel Hollow Fiber Bioreactor System
• Analysis of MAGE-3 derived synthetic peptide as a human lung cancer antigen recognized by cytotoxic T lymphocytes
• Induction of Tumor-Specific Cytotoxic T Lymphocytes from Regional Lymph Node Lymphocytes of Human Breast Cancer
• Methylamine Treatment Changes the Allocation of Carbohydrate to Roots in Rice Plants(ENVIRONMENTAL BIOTECHNOLOGY)
• Production of Poly-β-Hydroxybutyric Acid by Microorganisms Accumulated from River Water Using a Two-Stage Perfusion Culture System
• Growth and Adhesion of Osteoblast-Like Cells Derived from Neonatal Rat Calvaria on Calcium Phosphate Ceramics
• Initial Anchoring and Proliferation of Fibroblast L-929 Cells on Unstable Surface of Calcium Phosphate Ceramics
• Improvement of Filtration Performance of Stirred Ceramic Mambrane Reactor and Its Application to Rapid Fermentation of Lactic Acid by Dense Cell Culture of Lactococcus lactis
• A Dense Cell Culture System for Aerobic Microorganisms Using a Shaken Ceramic Membrane Flask with Surface Aeration
• A Dense Cell Culture System for Microorganisms Using a Shake Flask Incorporating a Porous Ceramic Filter
• Time-Dependent Variation of the Surface Sturcture of Bioceramics in Tissue Culture Medium and the Effect on Adhesiveness of Cells
• Preparation of Calcium-Strontium Apatite through Mechanochemical Method
• Microbial Production of Optically Active 1,2-Diols Using Resting Cells of Alcaligenes sp. DS-S-7G
• Fuzzy Control in Microbial Production of ML-236B, a Precursor of Pravastatin Sodium
• Morphology Control of Preculture during Production of ML-236B, a Precursor of Pravastrain Sodium, by Penicillium citrinum
• Effects of the Surface Wettability and Zeta Potential of Bioceramics on the Adhesiveness of Anchorage-Dependent Animal Cells
• Overproduction of Thermostable β-Galactosidase in Escherichia coli, Its Purification and Molecular Structure
• Isolation of a Bacterium Assimilating (R)-3-Chloro-1,2-Propanediol and Production of (S)-3-Chloro-1,2-Propanediol Using Microbial Resolution
• Reaction Properties of Catalytic Antibodies Encapsulated in Organo Substituted SiO_2 Sol-Gel Materials(BIOCHEMICAL ENGINEERING)
• Detoxification of Bisphenol A and Nonylphenol by Purified Extracellular Laccase from a Fungus Isolated from Soil(ENVIRONMENTAL BIOTECHNOLOGY)
• Catalytic Properties of Lipases Immobilized on Various Mesoporous Silicates(Microbiology & Fermentation Technology)
• Preparation and Catalytic Performance of Lipases Encapsulated in Sol-Gel Materials(Microbiology & Fermentation Technology)
• Nucleotide Sequence of the LacN Gene Encoding Thermostable β-Galactosidase of a Thermophilic Anaerobe, Strain NA10
• Application of a Microporous Glass-Ceramics with a Skeleton of CaTi_4(PO_4)_6 to Carriers for Immobilization of Enzymes
• Influence of Ethylamine on the Crystal Growth of Hydroxyapatite Crystals
• Calcium Phosphate Formation on Highly - oriented Collagen Fibrils
• The Adhesiveness and Growth of Anchorage-Dependent Animal Cells on Biocompatible Ceramic Culture Carriers
• Carinal Resection after Induction Bronchial Arterial Infusion for Locally Advanced Non-Small Cell Lung Cancer
• Establishment of an immortalized T-cell line from lung cancer tissue : phenotypic and functional analyses
• Control of Crystal Orientation of Hydroxyapatite by Impostition of a High Magnetic Field
• Cloning and Expression of a NAD^+-Dependent Xylitol Dehydrogenase Gene (xdhA) of Aspergillus oryzae(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Identification of a lung cancer antigen evading CTL attack due to loss of human leukocyte antigen (HLA) class I expression
• Distribution of Ytterbium (Yb) in Cells of Streptomyces sp. YB-1 Which Can Accumulate Yb, and Reusability of Cells and Cell Membrane as Bioadsorbent for Yb(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of an Extracellular β-Agarase from Bacillus sp.MK03
• Purification and Characterization of an Extracellular α-Neoagarooligosaccharide Hydrolase from Bacillus sp. MK03
• Ytterbium-Decreasing Streptomyces sp.and Its Naphthoquinone-Pigment Production in the Presence of Rare-Earth Elements
• Surfactant-free Preparation of Poly(lactic acid)/Hydroxyapatite Microspheres
• Isolation and Characterization of a Novel Equol-Producing Bacterium from Human Feces
• Accumulation of Yttrium by Variovorax paradoxus
• Intra-Species Diversity between Seven Bifidobacterium adolescentis Strains Identified by Genome-Wide Tiling Array Analysis
• Plasma Kinetics and Urine Profile of Ethyl Glucosides after Oral Administration in the Rat
• The Multiplicity of N-Glycan Structures of Bovine Milk 18kDa Lactophorin (Milk GlyCAM-1)
• 2Fa02 合成ガス資化性好熱性細菌Moorella thermoaceticaにおける遺伝子組換え技術の開発(遺伝子工学,一般講演)
• 1Ap21 Dehydrogenase (xdhA, xdhC, ladA) Homologous Genes Disrupted Mutants of Aspergillus oryzae KBN616 for Increased Xylitol Production
• 2Fa04 温度感受性プラスミドを用いたビフィズス菌の部位特異的相同組換えに関する研究(遺伝子工学,一般講演)
• 2Fa03 ビフィズス菌における温度感受性プラスミドの作製と効率的な遺伝子破壊への利用(遺伝子工学,一般講演)
• Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Differences in sensitivity to tumor-specific CTLs between primary and metastatic esophageal cancer cell lines derived from the same patient
• Successful treatment of lung adenocarcinoma in an 18-year-old man
• Disruption and Overexpression of Acid Phosphatase Gene (aphA) from a Miso Koji Mold, Aspergillus oryzae KBN630, and Characterization of the Gene Product
• Hydrolysis of Soybean 7S and 11S Globulins Using Bacillus subtilis
• Xylitol production by NAD^+-dependent xylitol dehydrogenase (xdhA)- and L-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• 2Cp11 Acquirement of tolerance to oxygen in Bifidobacterium longum by expression of catalase
• 4Ip27 Xylitol Production Using xdhA and ladA Homologous Gene Disrupted Mutants of Aspergillus oryzae KBN616
• The determination of a small amount of biological constituent by the use of chemiluminescence. VII. An attempt of cyclic immunoaffinity chromatography.
• Intrapleural chemotherapy improves the survival of non-small cell lung cancer patients with positive pleural lavage cytology

もっと見る 閉じる

スポンサーリンク