Morphology Control of Preculture during Production of ML-236B, a Precursor of Pravastrain Sodium, by Penicillium citrinum
スポンサーリンク
概要
- 論文の詳細を見る
In order to scale-up the production of ML-236B, a precursor of pravastratin sodium, by Penicillium citrinum H strain, a method for controlling mycelial morphology was developed to increase the number of free mycelia in the preculture process. The use of a high concentration preculture medium (HCP medium) instead of a light medium, MBG3-4 medium, caused the morphology in the preculture broth to change from pellet from to flamentous form. A new preculture process was developed in which HCP medium was used in the third and the fourth precultures. Using this process, the number of free mycelia increased 100-fold and ML-236B production increased 1.5-fold compared to that obtained using the conventional process. It was possible to stabilize the productivity of ML-236B by inoculating the production culture with a fixed number of free mycelia.
- 社団法人日本生物工学会の論文
- 1993-12-25
著者
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Fukui F
Showa Sangyo Co. Ltd. Ibaraki Jpn
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Fukui Fumio
Fermentation Research Laboratories Sankyo Co. Ltd.
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Matsukawa Hiroshi
Fermentation Research Laboratories Sankyo Co. Ltd.
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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HOSOBUCHI MASAHIKO
Fermentation Research Laboratory, Sanko Co. Ltd.,
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YOSHIKAWA HIROJI
Fermentation Research Laboratory, Sankyo Co. Ltd.,
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SUZUKI TOSHIRO
Fermentation Research Laboratories, Sankyo Co. Ltd.,
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Hosobuchi M
Fermentation Research Laboratories Sankyo Co. Ltd.
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Yoshikawa H
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Yoshikawa Hiroji
Fermentation Research Laboratories Sankyo Co. Ltd.
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Hosobuchi Masahiko
Fermentation Research Laboratories Sankyo Co. Ltd.
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