New Sulfated Oligosaccharides Produced by Pseudomonas β-Agarase from Gracilaria verrucosa Polysaccharide

概要

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Polysaccharide (partially sulfated agarose) with macrophage-stimulation activity, derived from Gracilaria verrucosa, was decomposed by two types of β-agarase (agarases II and IV) from Pseudomonas sp. O-148. The hydrolysates were fractionated with ethanol precipitation and anion-exchange chromatography. The resulting anionic oligosaccharides with sulfate groups were investigated by ^<13>C-NMR spectroscopy. While the spectra of oligosaccharides produced by agarase IV showed identical patterns with those by β-agarase I from Pseudomonas atlantica and indicated the location of a sulfated saccharide unit on the non-reducing end, another new type of saccharide was found in the products by agarase II. The novel oligosaccharides by agarase II had a neoagarobiose unit on their nonreducing end and had sulfated units internally. This indicated the novelty of agarase II in cleavage fashion.
社団法人日本農芸化学会の論文
1998-06-23