Morphology Study in Production of ML-236B, a Precursor of Pravastatin Sodium, by Penicillium citrinum

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概要

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• A high level of ML-236B productivity was obtained in a mechanically agitated aertobic culture of Penicillium citrinum when small pellets were formed. Size and concentration of pellet for production had to be optimized. In contrast, cultures with filamentous growth exhibited high viscosities and pseudoplastic flow behavior. Since the oxygen transfer coefficient decreased with increading viscosity, such cultures became oxgen-limited and resulted in low product yield. In a large scale fermentation process, several preculture steps are required. Factors influencing pellet formation under such conditions were investigated. The mean diameters of pelletc increased and their number decreased as preculturing was repeated. However, after more than three precultures, many small pellets reappeared. These small pellets were throught to be formed by the agglomeration of free mycelia which were derived from the original pellets. The number of free mycelia formed during preculturing depended upon the characteristics of the strains as well as the culture conditions. Strains which produced soft pellets (low density) tended to form more free mycelia than did strains which produced tight pellets (high density). It is believed that borh the number and size of the pellets in the production culture could be regulated by changing the number of free mycelia which were transferred to the production culture from the preculture.

• 社団法人日本生物工学会の論文
• 1993-12-25

著者

• Ogawa Kiyoshi

  Research Laboratory Sanwa Shurui Co.ltd.

• Ogawa K

  Research Laboratory Sanwa Shurui Co.ltd.

• HOSOBUCHI MASAHIKO

  Fermentation Research Laboratory, Sanko Co. Ltd.,

• YOSHIKAWA HIROJI

  Fermentation Research Laboratory, Sankyo Co. Ltd.,

• OGAWA KANEO

  Fermentation Research Laboratories Sankyo Co. Ltd.,

• Yoshikawa Hiroji

  Fermentation Research Laboratories Sankyo Co. Ltd.

• Hosobuchi Masahiko

  Fermentation Research Laboratories Sankyo Co. Ltd.

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• Intracellular Fatty Acid Formation and Alcohol Acetyl Transferase Gene Expression in Brewing Yeast (Saccharomyces cerevisiae) Treated with Heat Shock
• Fuzzy Control in Microbial Production of ML-236B, a Precursor of Pravastatin Sodium
• Morphology Control of Preculture during Production of ML-236B, a Precursor of Pravastrain Sodium, by Penicillium citrinum
• Enhanced Glycerol Production in Shochu Yeast by Heat-Shock Treatment is Due to Prolonged Transcription of GPD1
• Production of ML-236B, and Inhibitor of 3-Hydroxy-3-methylglutaryl CoA Reductase, by Penicillium citrinum : Improvements of Strain and Culture Conditions
• Morphology Study in Production of ML-236B, a Precursor of Pravastatin Sodium, by Penicillium citrinum
• Conversion of Fermentation Pathway of Streptomyces saganonensis from Herbicidin A to Ara-A
• Degeneration of Herbicidin A-Producer, Streptomyces saganonensis, and Strain Improvement

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