Production of ML-236B, and Inhibitor of 3-Hydroxy-3-methylglutaryl CoA Reductase, by Penicillium citrinum : Improvements of Strain and Culture Conditions
スポンサーリンク
概要
- 論文の詳細を見る
In order to increase the yield of ML-236B, an intermediate for pravastatin Na (an inhibitor of cholesterol synthesis) production, the improvements of an ML-236B producing strain of Penicillium citrinum, the medium composition, and the culture conditions were studied. A mutant strain S-5808, which produces ML-236B 20 times more than the original strain does in flask culture, was isolated. As the cells of S-5808 required a large amount of the carbon source substrate to produce a lot of ML-236B, a fed-batch culture method was developed. The continuous feeding of carbon source (glycerol or mixture of glycerol and maltose)was very effective for the increase of ML-236B production. ML-236B in the culture broth became oily with lowering of pH during cultivation, adhered to the cell surface, decreased the carbon source consumption and finally stopped the ML-236B production at a late period of fermentation. The addition of a polypropylene glycol type surfactant enhanced the ML-236B productivity. The addition of the surfactant released oily ML-236B from the cell surface, therefore oxygen uptake, carbon source consumption, and ML-236B production continued throughout the fermentation.
- 社団法人日本農芸化学会の論文
- 1993-09-23
著者
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Arai Masami
Exploratory Chemistry Research Laboratories Sankyo Co. Ltd.
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Arai M
Exploratory Chemistry Research Laboratories Sankyo Co. Ltd.
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Arai M
Division Of Materials Science And Engineering Graduate School Of Engineering Hokkaido University
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HOSOBUCHI MASAHIKO
Fermentation Research Laboratory, Sanko Co. Ltd.,
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YOSHIKAWA HIROJI
Fermentation Research Laboratory, Sankyo Co. Ltd.,
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Shioiri Tetsuya
Fermentation Research Laboratories, Sankyo Co., Ltd.
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Ohyama Jo
Fermentation Research Laboratories, Sankyo Co., Ltd.
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Arai Masatoshi
Fermentation Research Laboratories, Sankyo Co., Ltd.
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Iwado Seigo
Fermentation Research Laboratories, Sankyo Co., Ltd.
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Hosobuchi M
Fermentation Research Laboratories Sankyo Co. Ltd.
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Iwado Seigo
Fermentation Research Laboratories Sankyo Co. Ltd.
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Yoshikawa H
Lead Discovery Research Laboratories Sankyo Co. Ltd.
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Yoshikawa Hiroji
Fermentation Research Laboratories Sankyo Co. Ltd.
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Ohyama Jo
Fermentation Research Laboratories Sankyo Co. Ltd.
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Hosobuchi Masahiko
Fermentation Research Laboratories Sankyo Co. Ltd.
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Shioiri Tetsuya
Fermentation Research Laboratories Sankyo Co. Ltd.
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