A Dense Cell Culture System for Microorganisms Using a Shake Flask Incorporating a Porous Ceramic Filter
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概要
- 論文の詳細を見る
A novel reactor design incorporating a porous ceramic tubular membrane fitted inside a shake flask was developed for the dense cell culture of microorganisms on an ordinary shaker. The tubular membrane was effective in extracting the culture broth. The filtering performance was found to be enhanced by fitting the filter at the shoulder of the flask. Membrane fouling was prevented for long periods by waves created in the culture broth due to the shaking action, which cnstantly washed the membrane surface. Using this shake flask system, the concentration of Saccharomyces cerevisiae cell mas reached 235 g/l at 220 h with the feedings of fresh medium and extraction of the supernatant. The long-lasting and high permeability performance of he ceramic membane enabled increases in the concentration and productivity of viable cells to be readily achieved by continuously refreshing the culture supernatant.
- 社団法人日本生物工学会の論文
- 1997-08-25
著者
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Suzuki Takahiro
Department Of Chemical Engineering Science Yokohama National University
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OHASHI RYO
Department of Biological Science and Technology, Science University of Tokyo
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KAMOSHITA YUYA
Department of Biological Science and Technology, Science University of Tokyo
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Ohashi R
Sci. Univ. Tokyo Chiba Jpn
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Ohashi Ryo
Department Of Biological Science And Technology Science University Of Tokyo
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Kamoshita Y
Department Of Biological Science And Technology Science University Of Tokyo
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Kamoshita Yuya
Department Of Biological Science And Technology Science University Of Tokyo
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Suzuki Takahiro
Department Of Anesthesiology Surugadai Nihon University Hospital
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