Continuous Production and Separation of Ethanol without Effluence of Wastewater Using a Distiller Integrated SCM-Reactor System
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概要
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Continuous ethanol production without effluence of wastewater was carried out using a closed circulation system which integrated a cell retention culture system and a distillation system to separate ethanol. The stirred ceramic membrane reactor(SCMR), a jar fermentor fitted with asymmetric porous alumina ceramic membrane rods, was used for retaining high density of cells and extraction of the culture supernatant that was continuously sent to the distiller to evaporate ethanol. After the distillation process, the residual solution of the culture supernatant was returned to the SCMR via a heat exchanger. Two types of strains of Saccharomyces cerevisiae;a flocculating IR2 strain and a sake-brewing K901 strain, were cultured using the integrated fermentation system for continuous production of ethanol. When the ethanol concentration reached 60 g/l, the culture supernatant was extracted by filtration from the SCMR and sent to the distiller. During the repeated ethanol fermentation and recycling of the medium, cell concentration of K901 strain was increased to 236 g/l and the productivity of ethanol reached 13.1 g・l^<-1>・h^<-1> which was higher than the productivity of 12.5 g・l^<-1>・h^<-1> achieved by immobilized cells on calcium alginate beads(CAB). In addition, the productivity was further increased to 14.5 g・l^<-1>・h^<-1> by immobilized K901 cells on CAB retained in the SCMR. In contrast, the flocculating IR2 strain was found to accumulate into crusts of cells on the surface of the ceramic filter and thereby the productivity of ethanol considerably decreased because of the reduced filtration performance. These results showed that the long-lasting and high filtration performance was essential for the increase in the productivity of ethanol using the integrated system that consisted of the SCMR and the distiller.
- 社団法人日本生物工学会の論文
- 1998-08-25
著者
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Kishimoto Michimasa
Department of Chemistry and Materials Technology, Kyoto Institute of Technology
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Suzuki Takahiro
Department Of Chemical Engineering Science Yokohama National University
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OHASHI RYO
Department of Biological Science and Technology, Science University of Tokyo
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KAMOSHITA YUYA
Department of Biological Science and Technology, Science University of Tokyo
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Ohashi R
Sci. Univ. Tokyo Chiba Jpn
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Ohashi Ryo
Department Of Biological Science And Technology Science University Of Tokyo
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Kamoshita Y
Department Of Biological Science And Technology Science University Of Tokyo
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Kamoshita Yuya
Department Of Biological Science And Technology Science University Of Tokyo
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Kishimoto Michimasa
Department Of Biological Science Technology Scientific University Of Tokyo
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Kishimoto Mishimasa
Department Of Biological Science And Technology Science University Of Tokyo
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Kishimoto Michimasa
Department Of Biotechnology Faculty Of Engineering Osaka University
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Kishimoto Michimasa
Deparmtnet Of Biotechnology Osaka University
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Suzuki T
Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University
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So Tomoko
Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health
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So Tetsuya
Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health
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Kishimoto Michimasa
International Center Of Cooperative Research In Biotechnology Japan Osaka University
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Suzuki Takahiro
Department Of Anesthesiology Surugadai Nihon University Hospital
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