Proteome Analysis of Threonine-Limited Lysine Fermentation by Brevibacterium flavum Using Two-Dimensional Electrophoresis
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概要
- 論文の詳細を見る
Proteome analysis using two-dimensional electrophoresis (2-DE) was applied to characterize the physiological states of Brevibacterium flavum during threonine-limited lysine fermentation. Cell samples taken from batch culture under different physiological states were analyzed by 2-DE, and protein spots were identified by peptide mass fingerprinting. On differential analysis of 2-DE maps, the differences in physiological states of microorganisms were recognized at the protein level. The proteomic information obtained by 2-DE of culture samples provided a global and dynamic view of changes in the physiological state of B. flavum in response to environmental conditions.
- Society of Chemical Engineers, Japanの論文
著者
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Kishimoto Michimasa
Department of Chemistry and Materials Technology, Kyoto Institute of Technology
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Horiuchi Jun-ichi
Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology
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TADA KIYOSHI
Department of Biotechnology, Graduate School of Engineering, Osaka University
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Kishimoto Michimasa
Deparmtnet Of Biotechnology Osaka University
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Horiuchi Jun-ichi
Department Of Biotechnology And Environmental Chemistry Kitami Institute Of Technology
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KANNO TOHRU
Department of Chemical System Engineering, Kitami Institute of Technology
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KASUYA Satoshi
Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology
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Tada Kiyoshi
Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology
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Kanno Tohru
Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology
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