Production of Poly-β-Hydroxybutyric Acid by Microorganisms Accumulated from River Water Using a Two-Stage Perfusion Culture System

概要

論文の詳細を見る
The perfusion culture system using a shaken ceramic membrane flask (SCMF) was employed to accumulate microorganisms separated from river water and to produce poly-β-hydroxybutyric acid (PHB). Using a two-step culture method with a single SCMF, river microorganisms were cultured by separately feeding four representative carbon sources, n-propanol, lactic acid, methanol, and formic acid. After 140 h culture, the cell concentration and PHB content respectively reached 43 g/l and 35% when a propanol medium was fed. Using a two-stage perfusion culture with twin SCMFs, the seed cell mass was increased in the first SCMF and then supplied to the second flask for PHB production. As a consequence, the cellular PHB content rose to 51% in the second SCMF, while the cell concentration gradually increased to 25 g/l after 175 h perfusion culture. These results demonstrated the utility of the tw-stage perfusion culture system for developing a cheap means of producing PHB coincident with wastewater treatment.
社団法人日本生物工学会の論文
2000-01-25