Purification and Characterization of an Alkaline Manganese Peroxidase from Aspergillus terreus LD-1

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概要

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• We report that Aspergillus terreus LD-1 produces an extracellular ligninolytic enzyme, manganese peroxidase (MnP), that reacts under alkaline conditions. This MnP was purified 13.1-fold from the culture supernatant to elicit a single band upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The molecular weight of this MnP was estimated as either 43 kDa by SDS-PAGE or 44 kDa by gel permeation chromatography, suggesting a monomeric structure. The optimum pH and temperature of this MnP are 12.5 and 37 respectively. This MnP is stable in the pH range 11.0 to 12.5 and also up to 40 The K_m values of this MnP for hydrogen peroxide, 2,6-dimethoxyphenol (2,6-DMP) and Mn^<2+> were 320μM,20μM and 33 μM at pH 12.5, respectively. The activity of the MnP is completely inhibited by Hg^<2+>, Pb^<2+>, Ag^+ and lactate. On the other hand, the MnP is activated by oxalate, maleate and fumarate. Maleate at 5 mM increased the MnP activity 5-fold. EDTA at 1 mM inhibited the MnP activity completely, but this inhibition was not observed in the presence of 1 mM Fe^<2+>.

• 2002-04-25

著者

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• SUZUKI Tohru

  Molecular Genetics Reseaech Center

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• Kawai Keiichi

  Department Of Biotechnology Faculty Of Agriculture Gifu University

• KANAYAMA NOZOMI

  United Graduate School of Agricultural Science, Gifu University

• Kanayama Nozomi

  United Graduate School Of Agricultural Science Gifu University

• Suzuki Tohru

  Molecular Genetics Research Center Gifu University

• Kawai Keiichi

  Department of Biotechnology Faculty of Agriculture, Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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