A Cytoplasmic Xylanase (XynX) of Aeromonas caviae ME-1 Is Released from the Cytoplasm to the Periplasm by Osmotic Downshock

スポンサーリンク

概要

• 論文の詳細を見る

• Aeromonas caviae ME-1 is a multiple xylanase-preducing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, Samia cynthia pryeri. One of the xylanases produced by A. caviae ME-1, XynX (38 kDa, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. Generally, xylanases are extracellular or cell surface enzymes. However, XynX is not exported to the extracellular fluid by A. caviae ME-1 and an Escherichia colt transformant harboring the xynX gene. In this study, we investigated the intracellular localization of XynX in A. caviae ME-1 and an E. coli transformant. XynX was found in the cytoplasm when the cells were grown under normal culture conditions. However, XynX was released from the cytoplasm to the periplasm during osmotic downshock. This release of XynX in the E. coli transformant was blocked in the presence of gadolinium chloride, which has been reported to be an inhibitor of bacterial mechanosensitive channels.

• 社団法人日本生物工学会の論文
• 2003-05-25

著者

• 鈴木 徹

  岐阜大院・連農・生資科

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Suzuki Tohru

  Life Science Research Center, Gifu University

• USUI KENGO

  Department of Biotechnology, United Graduate School Agricultural Science, Department of Biotechnolog

• AKISAKA TOSHITAKA

  Department of Oral Anatomy, School of Dentistry, Asahi University

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• Suzuki T

  Ihara & Company Ltd.

• Suzuki Tohru

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Usui Kengo

  Department Of Biotechnology United Graduate School Agricultural Science Department Of Biotechnology

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Kawai K

  Fac. Appl. Biol. Sci. Gifu Univ.

• Kawai Keiichi

  School Of Health Sciences Faculty Of Medicine Kanazawa University

• Akisaka T

  Asahi University School Of Dentistry Department Of Anatomy

• Akisaka Toshitaka

  Department Anatomy Asahi University School Of Dentistry

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• Kawai Keiichi

  Fac. Appl. Biol. Sci. Gifu Univ.

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• Suzuki Tohru

  The United Graduate School Of Agricultural Science Gifu University

• Suzuki Tohru

  Life Science Research Center Gifu University

• Kamimura Kiyohisa

  Department Of Radiology Fujimoto Hayasuzu Hospital

• SUZUKI Tohru

  The United Graduate School of Agricultural Science, Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

関連論文

• 植物系廃棄物の有効利用を目的としたデータベース作成
• 2Dp17 LNA(locked nucleic acid)プライマーを用いた活性汚泥中の古細菌クローニング(遺伝子工学,核酸工学,一般講演)
• The reproducibility of deep-inspiration breath-hold ^F-FDG PET/CT technique in diagnosing various cancers affected by respiratory motion
• Usefulness of rCBF analysis in diagnosing Parkinson's disease : supplemental role with MIBG myocardial scintigraphy
• Assessment of diastolic function using 16-frame ^Tl gated myocardial perfusion SPECT : a comparative study of QGS2 and pFAST2
• Identification of Single Base-pair Mutation on uidA Gene of Escherichia coli O157:H7 by Peptide Nucleic Acids (PNA) Mediated PCR Clamping
• 超好気性活性汚泥(HAIAS)によるリグニン分解
• 超好気性活性汚泥(HAIAS)による生デンプン分解
• 超好気性グラニュール処理での難分解性物質の処理
• 2A15-4 超好気性活性汚泥(HAIAS)による生デンプン分解(環境工学,廃水処理技術,一般講演)
• 2A15-5 超好気性活性汚泥(HAIAS)によるリグニン分解(環境工学,廃水処理技術,一般講演)
• 2A16-1 超好気性グラニュール処理での難分解性物質の処理(HAIAS)(環境工学,廃水処理技術,一般講演)
• 1Dp06 超好気的処理のDNAマイクロアレイを用いた微生物群の解析(生体情報工学,遺伝子工学,核酸工学,一般講演)
• 329. ビフィズス菌の生産するD-xylose Tsomeraseの単離精製とその諸性質について
• Canine SPECT studies for cerebral amino acid transport by means of ^I-3-iodo-α-methyl-L-tyrosine and preliminary kinetic analysis
• 1H17-4 酵母の比較ゲノム解析(遺伝子工学,核酸工学,一般講演)
• 2Ha03 ヒト糞便由来の新規Equol産生菌の単離と諸性質について(分類・系統・遺伝学他,一般講演)
• Purification and Characterization of D-Xylose Isomerase from Bifidobacterium adolescentis
• D-Glucose Isomerase from Bifidobacterium adolescentis
• Feasibility of Induction Chemotherapy Using Bronchial Arterial Infusion for Locally Advanced Non-Small Cell Lung Cancer : A Pilot Study
• Successful Induction of Tumor-specific Cytotoxic T Lymphocytes from Patients with Non-small Cell Lung Cancer Using CD8O-transfected Autologous Tumor Cells
• Interleukin-12 enhances the antitumor activity of cytotoxic T lymphocytes immunodeficient mice
• Optimization of Kojic Acid Production Rate Using the Box-Wilson Method
• Xylanase I of Aeromonas caviae ME-1 Isolated from the Intestine of a Herbivorous Insect (Samia cynthia pryeri)
• Fluorescent X-ray imaging with synchrotron radiation for the evaluation of probes for biomedical functions : A pilot study on 4-bromo-DL-phenylalanine for cancer detection in mice
• Detection of maleate-induced Fanconi syndrome by decreasing accumulation of ^I-3-iodo-α-methyl-L-tyrosine in the proximal tubule segment-1 region of renal cortex in mice : a trial of separate evaluation of reabsorption
• Renal accumulation and excretion of radioiodinated 3-iodo-α-methyl-L-tyrosine
• Transcellular transport of radioiodinated 3-iodo-α-methyl-L-tyrosine across monolayers of kidney epithelial cell line LLC-PK_1
• Cloning and Expression of NAD^+-Dependent L-Arabinitol 4-Dehydrogenase Gene (ladA) of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• 2F11-4 Xylitol dehydrogense genes of Aspergillus oryzae
• A Cytoplasmic Xylanase (XynX) of Aeromonas caviae ME-1 Is Released from the Cytoplasm to the Periplasm by Osmotic Downshock
• Construction of deletion mutants of Shiga(-like) toxin genes(stx-1 and/or stx-2) on enterohemorrhagic Escherichia coli(O157 : H7).
• Nucleotide sequence analysis of Shiga(-like) toxins from an exterohemorrhagic Escherichia coli isolated from the gifu, Japan, outbreak.
• Cloning, Expression, and Characterization of a Family 52 β-Xylosidase Gene (xysB) of a Multiple-xylanase-producing Bacterium, Aeromonas caviae ME-1
• XynX, a Possible Exo-xylanase of Aeromonas caviae ME-1 that Produces Exclusively Xylobiose and Xylotetraose from Xylan
• Expression of xyrA Gene Encoding for D-Xylose Reductase of Candida tropicalis and Production of Xylitol in Escherichia coli
• Cloning and Expression of a 58-kDa Xylanase VI Gene (xynD) of Aeromonas caviae ME-1 in Escherichia coli Which is not Categorized as a Family F or Family G Xylanase
• Cloning and Expression of Xylanase I Gene (xynA) of Aeromonas caviae ME-1 in Escherichia coli
• Production of Xylitol from D-Xylose by Candida tropicalis : the Effect of D-Glucose Feeding
• Cloning and Sequencing of Two D-Xylose Reductase Genes (xyrA and xyrB) from Candida tropicalis
• Production of Worcestershire Sauce by a Trickle Bed Bioreactor Using Comb-Shaped Ceramics Carriers
• Purification, Characterization and Structure Analysis of NADPH-Dependent D-Xylose Reductases from Candida tropicalis
• Cadmium Resistance Acquirement by IS1 Transposition into Escherichia coli C600
• A Study of Yeast Adsorption on Ceramic Beads by Zeta Potential
• Expression of a Plasmid-Encoded Gene for Cadmium Resistance of Pseudomonas putida GAM-1 in Escherichia coli
• A Mini-Scale Mass Production and Separation System for Secretory Heterologous Proteins by Perfusion Culture of Recombinant Pichia pastoris Using a Shaken Ceramic Membrane Flask
• Associations between liver ^F fluoro-2-deoxy-D-glucose accumulation and various clinical parameters in a Japanese population : influence of the metabolic syndrome
• Affinity of Leuconostoc Phosphoglycerate Mutase for Blue Dextran-Sepharose
• Cerebral blood flow abnormalities induced by transient hypothyroidism after thyroidectomy : Analysis by Tc-99m-HMPAO and SPM96
• Serial change in ^I-MIBG myocardial scintigraphy in non-insulin-dependent diabetes mellitus
• Analysis of MAGE-3 derived synthetic peptide as a human lung cancer antigen recognized by cytotoxic T lymphocytes
• Rapid Selection of Nonhotspot Mutants among hisD^+ Revertants of Salmonella typhimurium TA98 in Ames Test by Peptide Nucleic Acid (PNA)-Mediated PCR Clamping(ENVIRONMENTAL BIOTECHNOLOGY)
• Processing of XynE (110-kDa) of Aeromonas caviae ME-1 to 72-kDa Xylanase in Eseherichia coli Transformant
• The Processing of High-Molecular-Weight Xylanase (XynE, 110kDa) from Aeromonas caviae ME-1 to 60-kDa Xylanase (XynE_60) in Escherichia coli and Purification and Characterization of XynE_60(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Production of Poly-β-Hydroxybutyric Acid by Microorganisms Accumulated from River Water Using a Two-Stage Perfusion Culture System
• Growth and Adhesion of Osteoblast-Like Cells Derived from Neonatal Rat Calvaria on Calcium Phosphate Ceramics
• Initial Anchoring and Proliferation of Fibroblast L-929 Cells on Unstable Surface of Calcium Phosphate Ceramics
• Heterogeneous Cell Renewal of Pancreas in Mice : [^3H]-thymidine Autoradiographic Investigation
• Proliferation and Functional Changes of Pancreatic Gastrin Cells in Neonatal Rat
• Comparison of 1-week and 2-week triple therapy with omeprazole, amoxicillin, and clarithromycin in peptic ulcer patients with Helicobacter pylori infection: results of a randomized controlled trial
• Mechanism of Chromiun (VI) Toxicity in Escherichia coli: Is Hydrogen Peroxide Essential in Cr (VI) Toxicity?
• Induction of Cadmium-resistance of Pseudomonas putida GAM-1(Microbiology & Fermentation Industry)
• Establishment of an immortalized T-cell line from lung cancer tissue : phenotypic and functional analyses
• Ce^-induced exopolysaccharide production by Bradyrhizobium sp. MAFF211645(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of an Alkaline Manganese Peroxidase from Aspergillus terreus LD-1
• Interaction of Lactobacillus plantarum Phosphoglycerate Kinase with Cibacron Blue F3GA
• 521 Rapid Batch Ethanol Fermentation from Sugarcane Molasses by a Highly Flocculating and Thermotolerant Saccharomyces Species
• Cloning and Expression of a NAD^+-Dependent Xylitol Dehydrogenase Gene (xdhA) of Aspergillus oryzae(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
• Predictive value of Tc-99m galactosyl human serum albumin liver SPECT on the assessment of functional recovery after partial hepatectomy : a comparison with CT volumetry
• Identification of a lung cancer antigen evading CTL attack due to loss of human leukocyte antigen (HLA) class I expression
• 2Ea09 Gene analysis of methanol dehydrogenase of Bradyrhizobium sp. CE-3
• Distribution of Ytterbium (Yb) in Cells of Streptomyces sp. YB-1 Which Can Accumulate Yb, and Reusability of Cells and Cell Membrane as Bioadsorbent for Yb(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
• Purification and Characterization of an Extracellular β-Agarase from Bacillus sp.MK03
• Purification and Characterization of an Extracellular α-Neoagarooligosaccharide Hydrolase from Bacillus sp. MK03
• Ytterbium-Decreasing Streptomyces sp.and Its Naphthoquinone-Pigment Production in the Presence of Rare-Earth Elements
• Molecular structure and gene analysis of Ce^-induced methanol dehydrogenase of Bradyrhizobium sp. MAFF211645(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Comparative Characterization of the oah2 Gene Homologous to the oah1 of Thermus thermophilus HB8
• A Modified Process for Soy Sauce Fermentation by Immobilized yeasts(Microbiology & Fermentation Industry)
• Interaction of Candida Pyruvate Kinase with Cibacron Blue
• Affinity Chromatography of Candida Pyruvate Kinase on Bule Dextran-Sepharose
• Isolation and Characterization of a Novel Equol-Producing Bacterium from Human Feces
• Accumulation of Yttrium by Variovorax paradoxus
• Intra-Species Diversity between Seven Bifidobacterium adolescentis Strains Identified by Genome-Wide Tiling Array Analysis
• Plasma Kinetics and Urine Profile of Ethyl Glucosides after Oral Administration in the Rat
• The Multiplicity of N-Glycan Structures of Bovine Milk 18kDa Lactophorin (Milk GlyCAM-1)
• ペプチド核酸(PNA)の新たな利用法
• 2Fa02 合成ガス資化性好熱性細菌Moorella thermoaceticaにおける遺伝子組換え技術の開発(遺伝子工学,一般講演)
• 1Ap21 Dehydrogenase (xdhA, xdhC, ladA) Homologous Genes Disrupted Mutants of Aspergillus oryzae KBN616 for Increased Xylitol Production
• 2Fa04 温度感受性プラスミドを用いたビフィズス菌の部位特異的相同組換えに関する研究(遺伝子工学,一般講演)
• 2Fa03 ビフィズス菌における温度感受性プラスミドの作製と効率的な遺伝子破壊への利用(遺伝子工学,一般講演)
• Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• The agreement of left ventricular function parameters between ^Tc-tetrofosmin gated myocardial SPECT and gated myocardial MRI
• Successful treatment of lung adenocarcinoma in an 18-year-old man
• Disruption and Overexpression of Acid Phosphatase Gene (aphA) from a Miso Koji Mold, Aspergillus oryzae KBN630, and Characterization of the Gene Product
• Hydrolysis of Soybean 7S and 11S Globulins Using Bacillus subtilis
• Xylitol production by NAD^+-dependent xylitol dehydrogenase (xdhA)- and L-arabitol-4-dehydrogenase (ladA)-disrupted mutants of Aspergillus oryzae(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• Development of genetic transformation and heterologous expression system in carboxydotrophic thermophilic acetogen Moorella thermoacetica(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
• 2Cp11 Acquirement of tolerance to oxygen in Bifidobacterium longum by expression of catalase
• 4Ip27 Xylitol Production Using xdhA and ladA Homologous Gene Disrupted Mutants of Aspergillus oryzae KBN616
• Molecular Cloning and Sequence Analysis of an Extracellular Protease from Four Bacillus subtilis Strains

もっと見る 閉じる

スポンサーリンク