The Multiplicity of N-Glycan Structures of Bovine Milk 18kDa Lactophorin (Milk GlyCAM-1)

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概要

• 論文の詳細を見る

• Lactophorin is a heat-stable phosphoglycoprotein, also known as milk glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1). Bovine 18 kDa lactophorin was purified by heparin affinity chromatography from cow’s milk whey. Its N-glycans were obtained by proteomic techniques, including two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), followed by in-gel digestion with peptide-N4-(N-acetyl-β-glucosaminyl)-asparagine amidase (PNGase F). The released N-glycans were derivatized with 2-aminopryridine (PA) and analyzed by matrix-assisted laser desorption ionization quadruple ion trap time of flight mass spectrometry (MALDI-QIT-TOF MS). Among the MS analyzed peaks, 15 peaks were found to be N-glycan molecules as detected by MS2 analysis. These glycans consisted of mono-sialylated bi-, tri-, and tetra-antennary complex-type N-glycans carrying Gal-GlcNAc (LacNAc) or GalNAc-GlcNAc (LacdiNAc) with and without core-fucose.

• 社団法人 日本農芸化学会の論文
• 2010-02-23

著者

• 鈴木 徹

  岐阜大院・連農・生資科

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• Suzuki Tohru

  Life Science Research Center, Gifu University

• 金丸 美敬

  Dep. Of Applied Life Sci. Gifu Univ.

• Kanamaru Yoshihiro

  Department Of Food Science Faculty Of Agriculture Gifu University

• Kanamaru Yoshihiro

  Department Of Food Science Faculty Of Applied Biological Science Gifu University

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• Yabe Tomio

  Faculty Of Applied Biological Sciences Gifu University

• Nohara Daisuke

  Faculty Of Engineering Gifu University

• Kanamaru Yoshihiro

  Faculty Of Applied Biological Sciences Gifu University

• INAGAKI Mizuho

  United Graduate School of Agricultural Science, Gifu University

• SUZUKI Tohru

  Faculty of Applied Biological Sciences, Gifu University

• Suzuki Tohru

  United Graduate School Of Agricultural Sci. Gifu Univ.

• NAKAYA Shuuichi

  Analytical and Measuring Instruments Division, Shimadzu Corporation

• Suzuki Tohru

  The United Graduate School Of Agricultural Science Gifu University

• Nakaya Shuuichi

  Analytical And Measuring Instruments Division Shimadzu Corporation

• Suzuki Tohru

  Molecular Genetics Research Center Gifu University

• Suzuki Tohru

  United Grad. Sch. Agr. Sci. Gifu Univ.:fac. Appl. Biol. Sci. Gifu Univ.

• Inagaki Mizuho

  Department Of Applied Life Science Gifu University

• Yabe Tomio

  Department Of Applied Life Science Gifu University

• Inagaki Mizuho

  Department of Applied Life Science, Gifu University

• SUZUKI Tohru

  The United Graduate School of Agricultural Science, Gifu University

• Yabe Tomio

  United Graduate School of Agricultural Science, Gifu University

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