Cloning and Expression of Xylanase I Gene (xynA) of Aeromonas caviae ME-1 in Escherichia coli

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概要

• 論文の詳細を見る

• A xylanase gene, xynA, which encodes xylanase I of Aeromonas caviae ME-1,was cloned in Escherichia coli, xynA gene has a 633 bp open reading frame (encoding 211 amino acid residues) which includes a signal peptide (28 amino acid residues). E. coli JM109 transformant produced 0.7 U/ml xylanase, about 80% of which was secreted into the culture medium as mature enzyme. The deduced amino acid sequence shows 79.3,78.9,and 47.1% identity to family G xylanases of Bacillus subtilis, Bacillus pumilus and Bacillus circulans, respectively.

• 社団法人日本生物工学会の論文
• 1997-03-25

著者

• SUZUKI Tohru

  United Graduate School of Agricultural Science, Gifu University

• Kawai K

  Faculty Of Applied Biological Sciences Gifu University

• TAKAMIZAWA Kazuhiro

  Department of Bioprocessing, Faculty of Agriculture, Gifu University

• Suzuki T

  Life Science Research Center Gifu University:faculty Of Applied Biological Sciences Gifu University

• NAITO Hiroshi

  Department of Artificial Organs, National Cardiovascular Center Research Institute

• SUZUKI TOHRU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

• Horitsu Hiroyuki

  Department ofBiotechnology, Division of Bioresources Utilization, Faculty ofAgriculture,Gifu Univ.

• Kawai Keiichi

  Department of Biotechnology, Division of Bioresources Utilization, Faculty of Agriculture, Gifu Univ

• Kubata Bruno

  United Graduate School Of Agricultural Science

• Takamizawa Kazuhiro

  Faculty of Applied Biological Sciences, Gifu University

• ITO YUKIHITO

  Department of Biotechnology, Faculty of Agriculture

• Ito Yukihito

  Department Of Biotechnology Faculty Of Agriculture

• Horitsu Hiroyuki

  Department Of Biotechnology Faculty Of Agriculture:(present Address)facculty Of Home Economics Depar

• Horitsu Hiroyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University

• Naito Hiroshi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Saito T

  Kanagawa Univ. Kanagawa Jpn

• Suzuki T

  Laboratory Of Microbial Biochemistry Institute For Chemical Research Kyoto University

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• Kawai Keiichi

  Faculty Of Applied Biological Sciences Gifu University

• Takamizawa K

  Faculty Of Applied Biological Sciences Gifu University

• Horitsu H

  Department Of Biotechnology Faculty Of Agriculture:(present Address)facculty Of Home Economics Depar

• Takamizawa Kazuhiro

  Department Of Agriculture Gifu University

• Naito Hiroshi

  Department Of Biotechnology Faculty Of Agriculture

• Kawai Keiichi

  Department Of Bioresources Utilization Faculty Of Agriculture Gifu University

• KAWAI Keiichi

  Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University

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