Cold-Active Lipolytic Activity of Psychrotrophic Acinetobacter sp. Strain No.6

概要

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A lipolytic bacterium, strain no.6, was isolated from Siberian tundra soil. It was a gram-negative coccoid rod capable of growing at 4℃ but not at 37℃ and was identified as a psychrotrophic strain of the genus Acinetobacter. Strain no.6 extracellularly produced a lipolytic enzyme that efficiently hydrolyzed triglycerides such as soybean oil during bacterial growth even at 4℃; it degraded 60% of added soybean oil (initial concentration, 1% w/v) after cultivation in LB medium at 4℃ for 7 d. Thus, the bacterium is potentially applicable to in-situ bioremediation or bioaugumentation of fat-contaminated cold environments. We partially purified the lipolytic enzyme from the culture filtrate by acetone fractionation and characterized it. The enzyme preparation contained a single species of cold-active lipase with significant activity at 4℃, which was 57% of the activity at the optimum temperature (20℃). The enzyme showed a broad specificity toward the acyl group (C_8-C_<16>) of substrate ethyl esters.
社団法人日本生物工学会の論文
2001-08-25