Identification of an inducible glucosyltransferase from Phytolacca americana L. cells that are capable of glucosylating capsaicin
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概要
- 論文の詳細を見る
Cell suspension cultures of Phytolacca americana L. (pokeweed) are capable of glycosylating capsaicinoids that have several biomedical applications. To identify the capsaicinoid glucosyltransferase involved in this biotransformation, we isolated three full-length cDNAs (PaGTs) encoding homologs of plant secondary product glycosyltransferases from cultured cells of P. americana L. These glycosyltransferase cDNAs were heterologously expressed in Escherichia coli cells and the expressed products were functionally characterized. Although all of these glycosyltransferases displayed broad glucosyl-acceptor specificities toward phenolics, capsaicinoid glucosyltransferase activity was found only for one of the cloned enzymes, PaGT3. Phylogenetic analysis showed that PaGT3 is the most closely related to betanidin 5-O-glucosyltransferase from Dorotheanthus bellidiformis, and in fact, it displayed a weak betanidin 5-O-glucosyltransferase activity. Transcription analyses showed that the expression of PaGT3 in P. americana L. was strongly induced by exposure of the cells to capsaicin (0.65 mM). These results show that PaGT3 should be, at least in part, responsible for the capsaicinoid glucosyltransferase activity of this plant.
- 日本植物細胞分子生物学会の論文
- 2009-06-01
著者
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西野 友年
神戸大理
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KWON Soonil
Department of Global Agricultural Sciences, The University of Tokyo
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Takahashi Seiji
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nishino Tokuzo
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nakayama Toru
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Nishino T
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Nishino Tokuzo
Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi
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Hosoya Miho
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Hemmi H
Tohoku Univ. Miyagi Jpn
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Hemmi Hisashi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Hemmi Hisashi
Department Of Biochemistry And Engineering Tohoku University
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Hemmi Hisashi
Dep. Of Biochemistry And Engineering Graduate School Of Engineering Tohoku Univ. Aoba-yama Sendai Mi
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NOGUCHI Akio
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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KUNIKANE Satoshi
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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HOMMA Hiroaki
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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LIU Wenhai
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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SEKIYA Takashi
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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OHIWA Shingo
Department of Life Science, Faculty of Science, Okayama University of Science
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KATSURAGI Hisashi
Sunny Health Co. Ltd.
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HAMADA Hiroaki
Department of Life Science, Faculty of Science, Okayama University of Science
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Nakayama Toru
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Takahashi Seiji
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Takahashi Seiji
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Kwon Soonil
Department Of Global Agricultural Sciences The University Of Tokyo
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Kwon Soonil
Department Of Life Science Faculty Of Science Okayama University Of Science
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Hamada Hiroaki
Department Of Life Science Faculty Of Science Okayama University Of Science
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Nishino Tokuzo
Department Of Biochemistry And Engineering Faculty Of Engineering Tohoku University
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Sekiya Takashi
Department Of Allergy And Rheumatology Faculty Of Medicine University Of Tokyo
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Liu Wenhai
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Ohiwa Shingo
Department Of Life Science Faculty Of Science Okayama University Of Science
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Noguchi Akio
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Kunikane Satoshi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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NAKAYAMA Toru
Department of Applied Microbial Technology, The Kumamoto Institute of Technology:(Present office)Institute for Fundamental Research, Suntory Ltd.
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HAMADA Hiroaki
Department of Biomolecular Engineering, Tokyo Institute of Technology
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KWON Soonil
Department of Applied Science, Faculty of Science, Okayama University of Science
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