Development of a Culture Carrier for Anchorage-Dependent Animal Cells Using Calcium Phosphate Ceramic Sinters

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概要

• 論文の詳細を見る

• Effect of the chemical and physical structure of bioactive ceramic sinters made by synthesized hydroxy-apatite (HAP) and tricalcium phosphate (TCP) on cell growth were studied to develop new carrier materials for cultivation of anchorage-dependent animal cells. A sophisticated analytic method using animal cells was used to characterize the affinity between the cells and ceramic sinters. In this method, cells were treated with trypsin for three minutes before culturing on ceramic plates. The initial anchoring cell ratio and the specific growth rate on the sintered plate were measured to compare the bioaffinity of ceramic materials. Using sintered mix-tures of HAP and TCP as culture carriers, the initial anchoring ration on the plates were reduced compared to a polystyrene culture dish (LUX) and a glass dish which were used as control test samples. The growth rate of cells was slightly accelerated on some of ceramic plates, however. The highest value of the specific growth rate, 0.049 (1/h), which was about 11% higher then the rate on the LUX plate, was obtained on the sinter which consists of a 4 : 1 mixture of HAP and TCP.

• 1990-09-25

著者

• So Tomoko

  Second Department Of Surgery Faculty Of Medicine University Of Occupational And Environmental Health

• So Tetsuya

  Second Department Of Surgery School Of Medicine University Of Occupational And Environmental Health

• KAWAMOTO YURI

  Department of Biotechnology, Graduate School of Engineering, Osaka University

• TORIYAMA MOTOHIRO

  Government Industrial Research Institute, Nagoya

• SUZUKI TAKAHIRO

  Government Industrial Research Institute, Nagoya

• KAWAMOTO YUKARI

  Government Industrial Research Institute, Nagoya

• YOKOGAWA YOSHIYUKI

  Government Industrial Research Institute, Nagoya

• Kawamoto Y

  Bioceramics Laboratory National Industrial Research Institute Of Nagoya

• Toriyama M

  Ceramics Research Institute National Institute Of Advanced Industrial Science And Technology

• Toriyama Motohiro

  Government Industrial Research Institute Nagoya

• KAWAMURA SUKEZO

  Government Industrial Research Institute

• Kawamoto Yuri

  Department Of Biotechnology Graduate School Of Engineering Osaka University

• Yokogawa Yoshiyuki

  National Institute Of Advanced Industrial Science And Technology (aist)

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