Application of a Microporous Glass-Ceramics with a Skeleton of CaTi_4(PO_4)_6 to Carriers for Immobilization of Enzymes
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概要
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A porous ceramics developed by controlled crystallization of glass in the CaO-TiO_2-P_2O_5 system (CTP) and subsequent acid leaching was found very durable in both acid and alkaline solutions because of the high TiO_2 content. The characteristics of CTP as an immobilizing carrier for various enzymes were investigated. Invertase, β-galactosidase, and alkalophilic proteinase were each immobilized to the porous ceramics, and the stabilities of the derivatives were observed in various pH conditions. The conventional inorganic carrier for enzymes, controlled pore glass (CPG), was used as a control test sample. The quantitative yield of β-galactosidase initially attached to CTP was about four times smaller compared to CPG. The stabilities of CTP derivatives in solutions of pH 4.6 and pH 9 were remarkably enhanced, however. Even in a neutral pH solution, the retained hydrolytic activity of β-galactosidase on CPG was rapidly lost at 37℃, while that of the CTP derivative was maintained for a long period. Since CPG is composed of 96% SiO_2,its derivatives dissolve into various pH solutions more rapidly than CTP. Our results showed that the new microporous ceramics is a more suitable material than the conventional silica glass carrieres to serve as an efficient and stable enzyme reactor for long term operations.
- 社団法人日本生物工学会の論文
- 1991-11-25
著者
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Hosono H
Erato-sorst Japan Science And Technology Agency (jst) In Frontier Research Center Tokyo Institute Of
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Hosono H
Nagoya Inst. Technology Nagoya
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Hosono Hideo
Institute For Molecular Science
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ABE YOSHIHIRO
Department of Public Health, Yamagata University Graduate School of Medicine
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Hosono Hideo
Department Of Materials Science And Engineering Nagoya Institute Of Technology
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TORIYAMA MOTOHIRO
Government Industrial Research Institute, Nagoya
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SUZUKI TAKAHIRO
Government Industrial Research Institute, Nagoya
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Toriyama Motohiro
Government Industrial Research Institute Nagoya
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Abe Yoshihiro
Department Of Materials Science And Engineering Nagoya Institute Of Technology
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Abe Yoshihiro
Department Of Fundamental Experimental Pharmaco-science Kyoritsu College Of Pharmacy
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Hosono Hideo
Department of Industrial Chemistry, Faculty of Technology, Tokyo Metropolitan University
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