Purification and Some Properties of Acid Invertase of Persimmon Fruits

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概要

• 論文の詳細を見る

• Single cells were prepared from mesocarp tissue of ripe persimmon (Diospyros kaki cv.Fuyu) fruits, and inter-or intracellular localization of acid invertase (AI, EC 3.2.1.26) was studied. AI was localized in the intercellular fraction (cell wall fraction). AI was isolated and purified from the cell wall fraction of ripe persimmon fruits by column chromatography on SE-53 cellulose and Toyopearl HW 55F. The specific activity of purified AI was 570 units per mg protein at 30℃. The molecular mass of AI was estimated to be 44 kDa by gel filtration over Sephacryl S-200 and 70 kDa by SDS-PAGE. The optimum pH of the activity for sucrose was 4.25. The purified enzyme hydrolyzed sucrose and raffinose but not melibiose. The enzyme had a K_m of 3.2 mM for sucrose and a K_m of 2.6 mM for raffinose. Silver nitrate (5μM), HgCl_2 (2μM), p-chloromercuribenzoate (100 mM), pyridoxamine (10mM), and pyridoxine (2.5 mM) inhibited AI activity by 95,85,100,41,and 30%, respectively.

• 社団法人日本農芸化学会の論文
• 1992-12-23

著者

• SATO Takahide

  Graduate School of Science and Technology, Chiba University

• Ogura N

  Division Of Bioproduction Science Faculty Of Horticulture Chiba University

• Ogura N

  Chiba Univ. Chiba Jpn

• Sato T

  Fac. Horti. Chiba Univ.

• Sato Takahide

  Graduate School Of Science And Technology Chiba University

• Nakagawa H

  Graduate School Of Science And Technology Chiba University

• Nakagawa Hiroki

  Fac.hort Chiba Univ.

• NAKAGAWA Hiroki

  Division of Bioproduction Science, Faculty of Horticulture, Chiba University

• OGURA Nagao

  Division of Bioproduction Science, Faculty of Horticulture, Chiba University

• SATO Takahide

  Division of Bioproduction Science, Faculty of Horticulture, Chiba University

• IWATSUBO Tomoyoshi

  Division of Bioproduction Science, Faculty of Horticulture, Chiba University

• TAKAHASHI Tatsuo

  Division of Bioproduction Science, Faculty of Horticulture, Chiba University

• Ogura N

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Sato Takenhito

  Department Of Immunology Tokai University School Of Medicine

• Iwatsubo Tomoyoshi

  Division Of Bioproduction Science Faculty Of Horticulture Chiba University

• Sato Tsutomu

  Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And

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