Isolation and molecular characterization of a SoWRKY1 transcription factor from spinach (Spinacia oleracea)
スポンサーリンク
概要
- 論文の詳細を見る
WRKY genes encode proteins which belong to a large family of transcription factors that are involved in various developmental and physiological processes, response to pathogen infections and wound stress in plants. The molecular characteristics of WRKY genes involved in these are becoming clear in model plants, like Arabidopsis. However, knowledge of WRKY genes in other plants, for instance vegetable, is still not enough. In the present studies, using a yeast one-hybrid system we isolated a cDNA from a spinach cDNA library and characterized its function. The cDNA, designated SoWRKY1, encodes a putative polypeptide of 362 amino acids which is highly homologous to Arabidopsis WRKY40 (AtWRKY40). The putative primary structure of SoWRKY1 contains a single WRKY domain of Cys2His2 zinc finger motif and a potential nuclear localization signal (NLS), whose structure is characteristic of group IIa type WRKY protein. SoWRKY1-sGFP fusion protein was localized to the nucleus when the protein was expressed in onion epidermal cells. SoWRKY1 showed high binding affinity to DNA molecules containing TTGAC(C/T) W-box sequences and transcriptional activation activity in yeast. SoWRKY1 transcripts in spinach leaves were transiently induced by wounding treatment and salicylic acid (SA). The transcripts accumulated following treatment with cycloheximide (CHX), a protein biosynthesis inhibitor. Functional analysis of SoWRKY1 in vivo was performed by overexpression in Arabidopsis, and pathogenesis-related PR1 and PR2 gene expression level increased in the transgenic plants. These results suggest that SoWRKY1 might be involved as a transcription factor in defense-related signaling transduction pathways of spinach.
- 2010-06-25
著者
-
HUANG Shengzhu
Graduate School of Science and Technology, Chiba University
-
YAMAMOTO Hiroyuki
Graduate School of Science and Technology, Chiba University
-
IDE Nobuyuki
Faculty of Horticulture, Chiba University
-
MIZUNO Shinji
Graduate School of Science and Technology, Chiba University
-
SHIRAISHI Naomasa
Graduate School of Agricultural Science, Kobe University
-
SATO Takahide
Graduate School of Science and Technology, Chiba University
-
NAKAGAWA Hiroki
Graduate School of Science and Technology, Chiba University
-
SONODA Masatoshi
Graduate School of Science and Technology, Chiba University
-
Sato T
Fac. Horti. Chiba Univ.
-
Sato Takahide
Graduate School Of Science And Technology Chiba University
-
Ide Nobuyuki
Faculty Of Horticulture Chiba University
-
Ide Nobuyuki
Faculty Of Applied Biological Science Hiroshima University
-
Mizuno Shinji
Graduate School Of Science And Technology Chiba University
-
Sato Takahide
Graduate School Of Science And Engineering Tokyo Institute Of Technology
-
Huang Shengzhu
Graduate School Of Science And Technology Chiba University
-
Nakagawa H
Graduate School Of Science And Technology Chiba University
-
Nakagawa H
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
-
Nakagawa Hiroki
Fac.hort Chiba Univ.
-
Nakagawa Hiroki
Graduate School Of Science And Technology Chiba University
-
Nakagawa Hiroki
Dep. Of Applied Biochemistry And Food Sciences Fac. Of Agriculture Saga Univ.
-
Sonoda Masatoshi
Graduate School Of Science And Technology Chiba University
-
Shiraishi Naomasa
Plant Nutrition Laboratory Faculty Of Agriculture Kobe University
-
Shiraishi Naomasa
Graduate School Of Agricultural Science Kobe University
-
Sato Takenhito
Department Of Immunology Tokai University School Of Medicine
-
Sato Tsutomu
Department Of Applied Biological Chemistry Faculty Of Agriculture And Graduate School Of Science And
-
Yamamoto Hiroyuki
Graduate School Of Bio-agricultural Sciences Nagoya University
-
Yamamoto Hiroyuki
Graduate School Of Science And Technology Chiba University
関連論文
- 繊維方向一軸応力を受ける木材のマクロなひずみと結晶格子ひずみとの関係
- 218 木材のX線応力測定(木質系材料の基礎物性-I,木材,その循環資材としての機能と課題,オーガナイスドセッション4,第53期学術講演会)
- 繊維方向繰り返し一軸引張応力を受けるヒノキ材のX線応力測定(木質材料)
- 211 等価介在物法を応用した木質細胞壁の力学挙動のモデル化とその実験的検証(組織と力学的挙動)
- A Nitrate Reductase Inactivator Protein from Spinach. Purification, Molecular Weight and Subunit Composition
- Isolation and molecular characterization of a SoWRKY1 transcription factor from spinach (Spinacia oleracea)
- Effects of Japanese Black Tea on Atherosclerotic Disorders
- Inhibitory Effect of Green Tea on Injury to a Cultured Renal Epithelial Cell Line, LLC-PK_1
- Inhibition of Proteasome by MG-132 Treatment Causes Extra Phragmoplast Formation and Cortical Microtubule Disorganization during M/G_1 Transition in Synchronized Tobacco Cells
- Overexpression of a gene for 26S proteasome subunit RPN10 confers enhanced resistance to canavanine, an analog of arginine, in transgenic rice (Oryza sativa L.)
- Cell-cycle-related variation in proteins in suspension-cultured rice cells
- Cell-Cycle Dependent Dynamic Change of 26S Proteasome Distribution in Tobacco BY-2 Cells
- RECONSTRUCTION ( in vitro ) OF 20S PROTEASOME AND REGULATORY SUBUNITS FROM THE CULTURED RICE ( Oriza sativa) CELLS
- Cloning and Sequencing of cDNA from Oryza sativa encoding a homolog to non-ATPase subunit,MBP1, of 26S Proteasome in Arabidopsis thaliana
- PURIFICATION AND CHARACTERIZATION OF A 26S PROTEASOME FROM THE CULTURED RICE (Oriza sativa)CELLS
- Changes in Proteasome Levels in Spinach (Spinacia oleracea) Seeds during Imbibition and Germination
- Ex vivo evidence for asymmetric tyrosine phosphorylation of ZAP-70 on double-positive thymocytes in the positive selection process
- Measurement of Nitrate Concentration Distribution in Vegetables by Near-Infrared Hyperspectral Imaging
- 熱帯早生樹の材質研究 : 用材化へ向けたフィールドワーク
- 早生樹の材質と成熟材形成
- 134 熱帯早生樹の成長速度が材成熟に及ぼす影響(木質バイオマス利用の新地平III,オーガナイズドセッション4.木質バイオマス利用の新地平)
- Estimating the equilibrium position by measuring growth stress in weeping branches of Prunus spachiana Kitamura f. spachiana cv. Plenarosea
- Negative gravitropism and growth stress in GA_3-treated branches of Prunus spachiana Kitamura f. spachiana cv. Plenarosea
- Relation between growth stress and lignin concentration in the cell wall Ultraviolet microscopic spectral analysis
- NEGATIVE GRAVITROPISM AND GROWTH STRESS IN GA_3-TREATED BRANCHES OF THE WEEPING TYPE OF JAPANESE CHERRY
- MECHANICAL CONTROL OF GROWTH PATTERN OF TREE SHOOT BY GROWTH STRESS GENERATION
- Design of Low Power Track and Hold Circuit Based on Two Stage Structure
- Multi-Path Analog Circuits Robust to Digital Substrate Noise
- High Speed Transconductance-C-Opamp Integrator Using Current-Feedback Amplifier(Building Block, Analog Circuit and Device Technologies)
- Design Optimization of Active Shield Circuits for Digital Noise Suppression Based on Average Noise Evaluation(Analog Circuit Techniques and Related Topics)
- Equivalent Saturated MOSFET Circuit with Wide Input Range(Analog Circuit Techniques and Related Topics)
- Optimized Design of Active Shield Circuit with Consideration on On-chip Layout
- Rail-to-Rail V-I Conversion Using a Pair of Single Channel MOSFETs Operating in Plural Regions
- Extension of Current Conveyor Concept and Its Applications
- Fully On-Chip Active Guard Band Circuit for Digital Noise Cancellation(Special Section on Analog Circuit Techniques and Relate)
- Wide-Input Range Linear Voltage-to-Current Converter Using Equivalent MOSFETs without Cutoff Region(Special Section on Analog Circuit Techniques and Related Topics)
- Design Optimization of Active Guard Band Circuit with Consideration on Device Matching and Frequency Characteristic
- High PSRR Active Guard Band Circuit For Digital Noise Suppression
- A Novel Design Strategy for Class A CMOS Second Generation Current Conveyors(Special Section on Analog Circuit Techniques Supporting the System LSI Era)
- Substrate Noise Suppression Using Active Guard Band Circuit
- A BiCMOS Seventh-Order Lowpass Channel-Select Filter Operating at 2.5V Supply for a Spread-Spectrum Wireless Receiver(Special Section on Analog Circuit Techniques and Related Topics)
- Zero Common-Mode Gain Fully Balanced Circuit Structure
- Design of an All-Pass Filter using a class AB 3-Input CMOS OTA
- CLONING AND CHARACTERIZATION OF AUXIN-RESPONSIVE ACC SYNTHASE GENES FROM MELON
- ETHYLENE FORMATION IN MELON FRUITS DURING THE EARLY STAGE OF RIPENING
- CLONING OF cDNAs FOR AUXIN INDUCED1-AMINOCYCLOPLOPANE-1-CARBOXYLATE (ACC) SYNTHASE FROM ETIOLATEDSEEDLINGS OF MELON
- ETHYLENE FORMATION IN SEEDS DURINGGROWING OF MELON FRUITS
- AUXIN-RESPONSE ELEMENTS IN MELON ACC SYNTHASE GENE (CMe-ACS2) PROMOTER
- CLONING AND EXPRESSION ANALYSIS OF NITRATE REDUCTASE INACTIVATOR (NRI) GENE FROM SPINACH
- The Synthesis of Ethylene in Melon Fruit during the Early Stage of Ripening : GROWTH AND DEVELOPMENT
- Intercellular Localization of Acid Invertase in Tomato Fruit and Molecular Cloning of a cDNA for the Enzyme
- Purification and Some Properties of Acid Invertase of Persimmon Fruits
- Acid Invertase of Melon Fruits : Immunochemical Detection of Acid Invertases : GROWTH AND DEVELOPMENT
- Control by Glutamine of the Synthesis of Nitrate Reductase in Cultured Spinach Cells : PROTEINS, ENZYMES AND METABOLISM
- Size and Levels of mRNA for Acid Invertase in Ripe Tomato Fruit
- Immunological Characterization of Nitrate Reductase in Different Tissues of Spinach Seedlings
- Polygalacturonase mRNA of Tomato : Size and Content in Ripe Fruits
- Cell-Free Synthesis of a Putative Precursor of Polygalacturonase in Tomato Fruits
- Effect of Heat Treatment on the Development of Polygalacturonase Activity in Tomato Fruit during Ripening
- Monoclonal Antibodies Spicific for NADH-Ferricyanide Reductase Domain of Spinach Leaf Nitrate Reductase
- Forward Subchannel Control Scheme for TDD Multi-Carrier Mobile Communication System (Special Section on Multi-dimensional Mobile Information Networks)
- CD45 can act as a negative regulator for the transition from early to late CD4^+ CD8^+ thymocytes
- Transcription and demethylation of TCR β gene initiate prior to the gene rearrangement in c-kit^+ thymocytes with CD3 expression: evidence of T cell commitment in the thymus
- Effects of a Component of Green Tea on the Proliferation of Vascular Smooth Muscle Cells
- Synthesis of Novel 26-Substituted Milbemycin A_4 Derivatives and Their Acaricidal Activities
- Synthesis and Insecticidal Activity of Novel N-Oxydihydropyrrole Derivatives with a Substituted Spirocyclohexyl Group(Organic Chemistry)
- Synthesis and Insecticidal Activity of N-Oxydihydropyrroles : 4-Hydroxy-3-mesityl-5, 5-dimethyl Derivatives with Various Substituents at the 1-Position(Organic Chemistry)
- Synthesis of Novel 25-Substituted Milbemycin A_4 Derivatives and Their Acaricidal Activity against Tetranychus urticae
- Efficient N-Sulfenylation of Dihydropyrrole Derivatives Using N-Sulfenylphthalimides
- Synthesis of N-Oxydihydropyrrole Derivatives
- Quantitative Anhydrous Mercaptolysis of Algal Galactans Followed by HPLC of Component Sugars
- Purification and Characterization of the Alkaline Serine Protease Produced by Bacillus sp.N4 Strain from Fish Skin Mucus
- Antigen Feeding Increases Frequency and Antigen-specific Proliferation Ability of Intraepithelial CD4^+ T Cells in αβ T Cell Receptor Transgenic Mice(Food & Nutrition Science)
- Ureide and Amino Acids in Xylem Sap of Soybean (Glycine max L.) Are Affected by Both Nodulation and Nitrogen Supply from Soil
- Ureide and Amino Acids in Xylem Sap of Soybean (Glycine max L.) Are Affected by Both Nodulation and Nitrogen Supply from Soil (Plant Nutrition)
- Nitrogen Fixation and Soil N Level during Maturation Affect the Contents of Storage Compounds of Soybean Seeds
- Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri
- Structural Characterization of the Carbohydrate Chains from the Skin Mucus Sialoglycoprotein of the Stingray Dasyatis akajei
- Isolation and Properties of a Sialoglycoprotein from the Skin Mucus of the Stingray Dasyatis akajei
- Evidence for down-regulation of highly expressed TCR by CD4 and CD45 on non-selected CD4^+CD8^+ thymocytes
- INVESTIGATION OF TRANSCRIPTION FACTOR OF NITRATE REDUCTASE
- PROMOTER ANALYSIS OF A SPINACH NITRATE REDUCTASE GENE
- Purification and characterization of nitrate reductase kinase (NR-Kinase) from Brassica camprestris L
- RECOMBINANT EXPRESSION OF SPINACH NITRATE REDUCTASE
- Improved CMOS Microwave Linearity Based on the Modified Large-Signal BSIM Model(Semiconductor Materials and Devices)
- Improved CMOS Microwave Linearity Based on the Modified Large-Signal BSIM Model
- New Estimation Method for Fatty Acid Composition in Oil Using Near Infrared Spectroscopy(Analytical Chemistry)
- Change in mechanical interaction between cellulose microfibril and matrix substance in wood cell wall induced by hygrothermal treatment
- Behavior of the cellulose microfibril in shrinking woods
- The influences of boiling and drying treatments on the behaviors of tension wood with gelatinous layers in Zelkova serrata
- Drag Reduction of Pipe Inlet Flow
- Active Shield Circuit for Digital Noise Suppression in Mixed-Signal Integrated Circuits(Analog Circuit Techniques and Related Topics)
- Effect of the lateral growth rate on wood properties in fast-growing hardwood species
- Reevaluation of the promoter structure of the class 3 flagellar operons of Escherichia coli and Salmonella
- Catalytic Function of the Residues of Phenylalanine and Tyrosine Conserved in Squalene-Hopene Cyclases(Organic Chemistry)
- Determination of three-dimensional viscoelastic compliance in wood by tensile creep test
- Selective Hydrolysis of the 3,6-Anhydrogalacotosidic Linkage in Red Algal Galactan : A Combination of Reductive Acid Hydrolysis and Anhydrous Mercaptolysis
- Functional Analyses of Tyr420 and Leu607 of Alicyclobacillus acidocaldarius Squalene-Hopene Cyclase. Neoachillapentaene, a Novel Triterpene with the 1, 5, 6-Trimethylcyclohexene Moiety Produced through Folding of the Constrained Boat Structure(Organic Che
- Heart splitting at crosscutting of eucalypt logs
- A Design of MOSFET-C Impedance Simulation Circuits Based on a GIC