Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol(Soil Biology)

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The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj_2Rj_3, Rj_3, Rj_4 and Rj_2Rj_3Rj_4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H'_β/H'_γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj_2-genotype and non-Rj, Rj_3 or Rj_4-genotype cultivars were significantly different. These results suggest that the Rj_2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.
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