Effects of Symbiosis with Rhizobium fredii on Transport of Fixed Nitrogen in the Xylem of Soybean Plants
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概要
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An experiment was conducted to identify the main nitrogenous compound transported in the xylem sap of soybean plants nodulated with Rhizobium fredii. Soybean (Glycine max L. Merr.) cultivars, wild type Bragg (nod^+, fix^+) and its nitrate tolerant, hypernodulating mutant nts1116 (nod^<++>, fix^+) were used for this experiment. These soybean plants were inoculated with a slow-growing rhizobium, Bradyrhizobium japonicum USDA110 or fast-growing rhizobia consisting of a mixture of R. fredii USDA191, USDA193, and USDA-194 and grown in a phytotron under natural light and controlled temperature conditions. Xylem sap was collected from Bragg and nts1116 plants at the flowering and pod elongation stages. Acetylene reduction activity per plant or per nodule weight was not different between soybean lines and inoculums. The composition of the nitrogenous compounds in the xylem sap was compared between the symbionts, with B. japonicum and R. fredii. At the flowering stage, ureide-N and amide-N accounted for 53 to 70% and 20 to 27% respectively of the total N in the sap collected from the plants inoculated either with B. japonicum or R. fredii. At the pod elongation stage, ureide-N and amide-N accounted for 74 to 85%, and 7 to 19% of total sap N. With the growth of the soybean plants, the ratio of ureide-N in the xylem sap increased. These results suggest that in the case of wild soybean and the hypernodulating mutant line nodulated by R. fredii, ureide is transported as the main nitrogenous compound of fixed nitrogen in the xylem sap in the same way as in plants nodulated with B. japonicum.
- 社団法人日本土壌肥料学会の論文
著者
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Yamakawa Takeo
Department Of Agricultural Chemistry Kyushu University
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Yamakawa T
Department Of Plant Resources Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu Un
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Ishizuka Junji
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Ishizuka Junji
Department Of Plant Resources Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu Un
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Shirai Teiko
Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu
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Shirai Teiko
Department Of Plant Resources Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu Un
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Yamakawa Takeo
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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