Detection of Plant Parasitic Nematodes, Meloidogyne incognita and Pratylenchus coffeae by Multiplex PCR Using Specific Primers (Soil Biology)
スポンサーリンク
概要
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We investigated the internal transcribed spacer (ITS) region of plant parasitic nematodes, namely the root-knot nematode, Meloidogyne incognita and root-lesion nematode, Pratylenchus coffeae that hamper the development of sustainable cropping systems in the southern region of Kyushu, Japan. In the current study, we attempted to detect M. incognita and P. coffeae using multiplex PCR using specific primer sets. M. incognita specific primer set (Mi1-Mi2) was designed based on the sequence of the ITS region of M. incognita. P. coffeae specific primer set (Pc1-Pc2) was designed according to both the report of Uehara et al. (Nematologica, 44: 357-368, 1998) and the sequence of P. coffeae obtained in this study. The use of multiplex PCR enable to detect two distinctive bands derived from the two kinds of plant parasitic nematodes. Furthermore, these two specific bands were detectable, when the genome of other nematodes was mixed in the template, with at least one nematode of the plant parasitic nematodes, M. incognita and P. coffeae being present with hundreds of other soil nematodes.
- 社団法人日本土壌肥料学会の論文
著者
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Saeki Yuichi
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Saeki Yuichi
Department Of Agriculture And Forest Science Faculty Of Agriculture Miyazaki University
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AKAO Shoichiro
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
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Saeki Yuichi
Dep. Of Biochemistry And Applied Biosciences Fac. Of Agriculture Miyazaki Univ.
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Saeki Y
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Nagatomo Yoshitaka
Department of Biochemistry and Applied Bioscience, Faculty of Agriculture, Miyazaki University
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Nagatomo Y
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Akao S
National Institute Of Agrobiological Resources
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Akao S
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Sato A
Faculty Of Agriculture Miyazaki University
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Akao Shoichiro
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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Saeki Yuichi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Akao Shoichiro
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture University Of Miyazaki
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Kawano Eiji
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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Yamashita Chigusa
Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, Miyazaki University
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Kawano Eiji
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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Yamashita Chigusa
Department Of Biochemistry And Applied Biosciences Faculty Of Agriculture Miyazaki University
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