Community Analysis of Seed-Associated Microbes in Forage Crops using Culture-Independent Methods

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概要

• 論文の詳細を見る

• Microbial diversity in forage crop seeds was examined using culture-independent methods. Environmental DNA samples were extracted from the surface of the seeds, and the intergenic spacer region between small subunit and large subunit RNA genes was then amplified by ribosomal intergenic spacer analysis (RISA) for the profiling of microbial community structures. The results suggested the presence of stable microbial communities in seeds and sequencing of the RISA amplicons identified a total of 33 unique microbial sequences in 1.6g of seed material from Italian ryegrass (Lolium multiflorum) and timothy (Phleum pretense). These included several sequences showing high similarity to known plant-associated microbes, such as Pseudomonas fluorescens and Clavibacter michiganensis, the Cladosporium and Dioszegia group of fungi, and also several uncultured bacteria.

• 日本微生物生態学会の論文

著者

• MINAMISAWA KIWAMU

  Graduate School of Life Sciences, Tohoku University

• SATO Toshiro

  Faculty of Engineering, Shinshu University

• Ezura Hiroshi

  Gene Research Center University Of Tsukuba

• Sato Toshiro

  Faculty Of Bioresource Sciences Akita Prefectural University

• Ezura Hiroshi

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Ikeda Seishi

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• Ikeda Soichiro

  Aspex Division Asahi Glass Co. Ltd.

• YTOW NOZOMI

  Gene Research Center, University of Tsukuba

• FUJI Shin-ichi

  Faculty of Bioresource Science, Akita Prefectural University

• Ikeda Seishi

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• FUJIMURA TATSUHITO

  Gene Research Center, University of Tsukuba

• Fujimura Tatsuhito

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Fuji Shin-ichi

  Faculty Of Bioresource Sciences Akita Prefectural University

• Fuji Shin-ichi

  Faculty Of Bioresource Science Akita Prefectural University

• Minamisawa Kiwamu

  Graduate School Of Life Sciences Tohoku Univ.

• Ytow Nozomi

  Gene Research Center University Of Tsukuba

• Fujimura Tatsuhito

  Nippon Del Monte Corp.

• Ytow N

  Gene Research Center University Of Tsukuba

• Ikeda Seishi

  Hokkaido Agricultural Research Center National Agriculture And Food Research Organization

• Minamisawa Kiwamu

  Graduate School of Life Science, Tohoku University

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