Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives
スポンサーリンク
概要
- 論文の詳細を見る
Protoplast culture and shoot regeneration from protoplast-derived calli were compared among different organs of Solanum integrifolium and three of its wild relatives (S. abutiloides, S. scabrum, and S. toxicarium). Leaves, cotyledons, and hypocotyls were used as sources of protoplast preparation. After one month of culture, a high frequency of visible colony formation was obtained from cotyledon protoplasts of S. integrifolium and S. scabrum, hypocotyl protoplasts of S. integrifolium and S. abutiloides, and leaf protoplasts of S. integrifolium and S. toxicarium. In addition, when the primary culture was started at a density of 2.5−5×104 protoplasts ml−1, the highest frequency of colony formation was obtained. Moreover, when the colonies were subcultured for 7 days on solid callus-proliferation medium before being transferred to shoot-induction medium, the plant regeneration frequency improved to between 91.8 and 98.8%.
- 日本植物細胞分子生物学会の論文
- 2006-12-01
著者
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EZURA Hiroshi
Graduate School of Life and Environmental Sciences, University of Tsukuba
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Iwamoto Yuzuri
Agricultural Food And Environmental Sciences Research Center Of Osaka Prefecture
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Iwamoto Yuzuri
Faculty Of Agriculture Niigata University
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Ezura Hiroshi
Graduate School Of Life And Environmental Sci. Univ. Of Tsukuba
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Ezura Hiroshi
Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba
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Iwamoto Yuzuri
Fac. Of Agriculture Niigata Univ.
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