Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses

スポンサーリンク

概要

• 論文の詳細を見る

• Thirteen glutamate receptor-like genes have recently been identified in tomato plants; however, their functions have not been fully elucidated. We overexpressed SlGLR1.1 and SlGLR3.5 in Arabidopsis and found that transgenic plants showed symptoms such as curled and deformed leaves, dwarf stature and retarded growth resembling those of Ca2+ deficiency. The results revealed that the levels of Ca2+ in aerial tissues did not differ between wild-type and transgenic plants, suggesting that overexpression of SlGLR1.1 and SlGLR3.5 did not affect Ca2+ uptake. Transgenic lines were hypersensitive to K+ and Na+ ionic stresses, which was rescued by addition of Ca2+ to the growth medium. Ectopic expression of SlGLR1.1 and SlGLR3.5 resulted in reduced efficiency of Ca2+ utilization, suggesting that these genes may play a role in calcium assimilation in tomato plants by controlling ionic transport across the membrane.

著者

• Ariizumi Tohru

  Department Of Crop And Soil Science Washington State University

• Ezura Hiroshi

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• AOUINI Asma

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• ARIIZUMI Tohru

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• MATSUKURA Chiaki

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• ASAMIZU Erika

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• Hernould Michel

  UMR 619 Physiology and Plant Biotechnology, National Institute of Agronomical Research, Bordeaux Universities 1 et 2

• Aouini Asma

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Hernould Michel

  Umr619 Physiology And Plant Biotechnology National Institute Of Agronomical Research Bordeaux Universities 1 Et 2 Research Centre

関連論文

• Gene trap strategy, an effective tool for identification of novel genes expressed in anther tissues in Arabidopsis thaliana
• Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits
• Gene dosage and genetic background affect miraculin accumulation in transgenic tomato fruits
• Efficient selection of a high-yield line by using somaclonal variation in Japanese butterbur (Petasites japonicus)
• Fertile somatic hybrids between Solanum integrifolium and S. sanitwongsei (syn. S. kurzii) as candidates for bacterial wilt-resistant rootstock of eggplant
• Abolition of the Tapetum Suicide Program Ruins Microsporogenesis
• Transgenic strawberry expressing the taste-modifying protein miraculin
• A Highly Efficient Transformation Protocol for Micro-Tom, a Model Cultivar for Tomato Functional Genomics
• Isolation of suppressors of late flowering and abnormal flower shape phenotypes caused by overexpression of the SHORT VEGETATIVE PHASE gene in Arabidopsis thaliana
• Efficacy of a special screened greenhouse covered by duplex fine mesh in reducing maize outcrossing
• Effect of a special screened greenhouse covered by fine mesh on maize outcrossing
• Reducing gene flow from pollen dispersal of genetically modified plants in special screened greenhouses
• Tomato genomics by JSOL
• Ethylmethanesulfonate (EMS) mutagenesis of Solanum lycopersicum cv. Micro-Tom for large-scale mutant screens
• NBRP, National Bioresource Project of Japan and plant bioresource management
• Microbial Diversity in Milled Rice as Revealed by Riosomal Intergenic Spacer Analysis
• Community Analysis of Seed-Associated Microbes in Forage Crops using Culture-Independent Methods
• Microbial Community Analysis of the Phytosphere Using Culture-Independent Methodologies
• Analysis of Molecular Diversity of Bacterial Chitinase Genes in the Maize Rhizosphere Using Culture-Independent Methods
• Microbial Community Analysis in the Rhizosphere of a Transgenic Tomato that Overexpresses 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase
• Discrimination of the Commercial Seeds of Forage Crops using Ribosomal Intergenic Spacer Analysis
• Soil microbial community analysis in the environmental risk assessment of transgenic plants
• Application of Ribosomal Intergenic Spacer Analysis to the Characterization of Microbial Communities in Rhizospheric Soils of the Bt Transgenic Chrysanthemum
• Transgenic Lotus japonicus with an Ethylene Receptor Gene Cm-ERS1/H70A Enhances Formation of Infection Threads and Nodule Primordia
• Ultrastructural Characterization of Exine Development of the transient defective exine 1 Mutant Suggests the Existence of a Factor Involved in Constructing Reticulate Exine Architecture from Sporopollenin Aggregates
• Cultivation under salt stress increases the concentration of recombinant miraculin in transgenic tomato fruit, resulting in an increase in purification efficiency
• Efficient plant regeneration from protoplasts of eggplant rootstock cultivar and its wild relatives
• Production of male sterile transgenic plants
• SOLANACEAE GENOMICS, SYSTEMS APPROACH TO DIVERSITY AND ADAPTAION
• Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
• TOMATOMA : A Novel Tomato Mutant Database Distributing Micro-Tom Mutant Collections
• Cultivation under salt stress increases the concentration of recombinant miraculin in transgenic tomato fruit, resulting in an increase in purification efficiency
• Prolonged exposure to atmospheric nitrogen dioxide increases fruit yield of tomato plants
• Tomato TILLING Technology: Development of a Reverse Genetics Tool for the Efficient Isolation of Mutants from Micro-Tom Mutant Libraries
• Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life
• Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca2+ utilization and promotes hypersensitivity to Na+ and K+ stresses
• Overexpression of the tomato glutamate receptor-like genes SlGLR1.1 and SlGLR3.5 hinders Ca^ utilization and promotes hypersensitivity to Na^+ and K^+ stresses
• Comparative analysis of common genes involved in early fruit development in Tomato and Grape
• Investigating the role of vitamin C in tomato through TILLING identification of ascorbate-deficient tomato mutants
• Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato
• Inhibition of CUTIN DEFICIENT 2 Causes Defects in Cuticle Function and Structure and Metabolite Changes in Tomato Fruit
• Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

もっと見る 閉じる

スポンサーリンク