Microbial Community Analysis in the Rhizosphere of a Transgenic Tomato that Overexpresses 3-Hydroxy-3-methylglutaryl Coenzyme A Reductase

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概要

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• The impact of overexpression of the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene on microbial diversity in the rhizosphere of a transgenic tomato was examined using a ribosomal intergenic spacer analysis (RISA) and a terminal restriction fragment length polymorphism (T-RFLP) analysis of two functional bacterial genes (bacterial chitinase and nifH genes). While the overall profiles of both RISA and T-RFLP revealed high similarity between the transgenic and non-trangenic tomato, several polymorphic DNAs were detected as differential bands in both the qualitative and quantitative evaluations. Sequence analysis suggested that differential bands cloned in RISA showed low levels of similarity to known species but were most likely derived from uncultured microbes. The phylogenetic analyses of two differential bands cloned in the T-RFLP analysis of the chitinase and nifH genes indicated that these bands belonged to Streptomyces like chitinase group and cyanobacterial nifH group, respectively.

• 日本微生物生態学会の論文

著者

• Komaki Hisayuki

  NITE Biotechnology Development Center (NBDC), Department of Biotechnology, National Institute of Tec

• MINAMISAWA KIWAMU

  Graduate School of Life Sciences, Tohoku University

• Ezura Hiroshi

  Gene Research Center University Of Tsukuba

• Ezura Hiroshi

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Ikeda Seishi

  Gene Research Center, Graduate School of Life and Environmental Sciences, University of Tsukuba

• Ikeda Soichiro

  Aspex Division Asahi Glass Co. Ltd.

• Komaki Hisayuki

  Nite Biological Resource Center (nbrc) National Institute Of Technology And Evaluation (nite)

• YTOW NOZOMI

  Gene Research Center, University of Tsukuba

• Ikeda Seishi

  Gene Research Center Graduate School Of Life And Environmental Sciences University Of Tsukuba

• FUJIMURA TATSUHITO

  Gene Research Center, University of Tsukuba

• OMURA Toshikazu

  Graduate School of Life and Environmental Sciences, University of Tsukuba

• Fujimura Tatsuhito

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Omura Toshikazu

  Univ. Tsukuba Ibaraki Jpn

• Omura Toshikazu

  Graduate School Of Life And Environmental Sciences University Of Tsukuba

• Minamisawa Kiwamu

  Graduate School Of Life Sciences Tohoku Univ.

• Ytow Nozomi

  Gene Research Center University Of Tsukuba

• Fujimura Tatsuhito

  Nippon Del Monte Corp.

• Ytow N

  Gene Research Center University Of Tsukuba

• Ikeda Seishi

  Hokkaido Agricultural Research Center National Agriculture And Food Research Organization

• Minamisawa Kiwamu

  Graduate School of Life Science, Tohoku University

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