Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development(Soil Biology)

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概要

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• During nodule development of legumes, together with the morphological alteration of plant cells, the rhizobia undergo marked biochemical and physiological changes and differentiate to bacteroids. Bacteroids invading a nodule are no longer free-growing cells, instead depending on the plant cell for all resources. To elucidate the molecular mechanism of the bacteroid differentiation of Bradyrhizobium japonicum, a time-course analysis of bacteroid protein profiles was studied by 2-D gel electrophoresis in soybean nodules. Using proteomic analysis, protein expressions in soybean nodule bacteroids 7, 10, 14, 28, and 49 days after inoculation (DAI) were monitored. The time points coincide with the early stage of nodule formation (7-10 DAI), the onset of nitrogen fixation (14-28 DAI), and nodule senescence (49 DAI). In this study, 275 annotated protein spots were successfully identified, and a cluster analysis of their expression was performed. A large portion of putative, upregulated proteins were observed in bacteroids at 7 or 10 DAI, and these upregulated proteins were mainly related to transcription, translation, protein folding, and degradation. In the later period (14-28 DAI) of bacteroid differentiation, a number of Nif and Fix proteins were upregulated. In addition, proteins related to the chaperonin and a synthetic enzyme of the poly-beta-hydroxybutyrate were expressed at high abundance. A number of proteins related to solute transporter were upregulated in the bacteroids and dominantly detected throughout nodule development. Changes of relative abundance of these proteins are discussed in relation to symbiosis.

著者

• Le Hoa

  Faculty Of Biology Hanoi University Of Education

• Kato Takahiko

  Department Of Chemistry And Bioscience Faculty Of Science Kagoshima University

• SATO Shusei

  Kazusa DNA Research Institute

• KATO Tomohiko

  Kazusa DNA Research Institute

• TABATA Satoshi

  Kazusa DNA Research Institute

• Dao Tan

  Faculty of Agriculture, Kagawa University

• Nomura Mika

  Faculty of Agriculture, Kagawa University

• Tajima Shigeyuki

  Faculty of Agriculture, Kagawa University

• KURIYAMA Tomoko

  RIKEN Plant Science Center

• Tabata Satoshi

  Kazusa Dna Res. Inst. Chiba Jpn

• 柿本 辰男

  Department Of Biology Graduate School Of Science Osaka University

• Kaneko Takakazu

  Kazusa Dna Research Institute

• Kaneko Takakazu

  Department Of Life Science Faculty Of Agriculture Kagawa University

• Kinoue Tomoyo

  Kagoshima Univ. Kagoshima Jpn

• Sato S

  Kazusa Dna Res. Inst. Chiba Jpn

• Tabata S

  Kazusa Dna Res. Inst. Chiba Jpn

• TAJIMA Shigeyuki

  Kagawa Univ.

• Dao Tan

  Kagawa Univ. Kagawa Jpn

• Nomura Mika

  Faculty Of Agriculture Kagawa University

• Nomura Mika

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Nomura M

  Kansai Electric Power Co. Inc. Amagasaki Jpn

• Nomura Mika

  Fac. Agr. Kagawa University

• Nomura Mika

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Tabata Satoshi

  Kazusa Dna Res.

• Tajima Shigeyuki

  Kagawa Univ. Kagawa Jpn

• Tajima Shigeysuki

  Department Of Life Science Faculty Of Agriculture Kagawa University

• Kato Tomohiko

  Mitsui Plant Biotech. Res. Inst.

• Sato Shusei

  Kazusa Dna Institute

• Arunothayanan Hatthaya

  Faculty of Agriculture, Kagawa University

• Tanaka K

  Kazusa Dna Research Institute

• Tanaka Kan

  Laboratory Of Molecular Genetics Department Of Molecular Biology Institute Of Molecular And Cellular

• Tanaka K

  Tokyo Metropolitan Inst. Medical Sci. Tokyo

• Kato Takahiko

  Lab. Of Molecular Microbiology School Of Agriculture Nagoya Univ.

• Tajima Shigeyuki

  Faculty Of Agriculture Kagawa University

• Tajima S

  Faculty Of Agriculture Kagawa University

• Tajima S

  Department Of Life Science Kagawa University

• Tajima Shigeyuki

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Dao Tan

  Faculty Of Agriculture Kagawa University:centre For Resources And Environmental Studies Vietnam Nati

• Nomura M

  Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Fac. Agr. Kagawa University

• Tajima Shigeyuki

  Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University

• Shinozaki Satoshi

  Research And Development Center Mayekawa Mfg. Co. Ltd.

• Arunothayanan Hatthaya

  Faculty Of Agriculture Kagawa University

• Takeda S

  Osaka Women's Univ. Osaka Jpn

• Tajima Shigeyuki

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Takegawa Kaoru

  Department Of Life Science Faculty Of Agriculture Kagawa University

• Nomura Mika

  Faculty Of Agriculture Kagawa University Kagawa

• Kiba T

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Kiba Takatoshi

  Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University

• Sayama Takashi

  National Agricultural Research Center For Hokkaido Region

• Takeda S

  Kazusa Dna Research Institute

• Kaneko Takakazu

  Kazusa Dna Res. Inst.

• Tabata S

  Kazusa Dna Res. Inst. Chiba

• Tajima Shigeyuki

  Dept. Bioresource Sience Kagawa Univ.

• Sawai Satoru

  Department Of Applied Biological Sciences Nihon University

• Tabata Satoshi

  Kazusa Dna Institute

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