Expression of Two Uricase (Nodulin-35) Genes in a Non-Ureide Type Legume, Medicago sativa

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概要

• 論文の詳細を見る

• Two cDNA clones, MsU2 and MsU9 encoding uricase (EC 1.7.3.3, Nodulin-35) were isolated from a cDNA library prepared from nodule tissues of alfalfa, Medicago sativa, plants. Both MsU2 and MsU9 encoded 308 amino acid polypeptides with a difference of 5 amino acids, and the deduced amino acid sequences shared 98% homology. Between these two cDNA clones and uricase genes of soybean which were designated as Nod-35s, more than 80% identity was observed in nucleotides and deduced amino acid sequences, suggesting that these MsU2 and MsU9 are homologs of Nod-35. Using the reverse transcription-PCR technique, we detected the transcripts of these two genes in almost all tissues of alfalfa. The operation of uricase genes was confirmed by the presence of ureide in the xylem sap and uricase activity in the nodules. In situ hybridization analysis revealed that MsU2 and MsU9 were expressed only in uninfected cells of the infected zone of the nodule tissue. The cell specific-expression of the two uricase genes was observed in an identical manner to that of Nod-35 in soybean nodules.

• 日本植物生理学会の論文

著者

• Nomura Mika

  Faculty of Agriculture, Kagawa University

• Tajima Shigeyuki

  Faculty of Agriculture, Kagawa University

• Cheng Xian-guo

  Faculty Of Agriculture Kagawa University:institute Of Soils And Fertilizers Chinese Academy Of Agric

• Nomura Mika

  Faculty Of Agriculture Kagawa University

• Nomura Mika

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Nomura M

  Kansai Electric Power Co. Inc. Amagasaki Jpn

• Nomura Mika

  Fac. Agr. Kagawa University

• Kouchi H

  National Inst. Agrobiological Sci. Ibaraki Jpn

• Kouchi Hiroshi

  Nati. Inst. Agrobiol. Res.

• Kouchi Hiroshi

  Natio. Insti. Agrobiol. Res. Tsukuba Naist

• Kouchi Hiroshi

  National Institute Of Agrobiological Resources Tsukuba Science City

• Tajima Shigeyuki

  Kagawa Univ. Kagawa Jpn

• Tajima Shigeysuki

  Department Of Life Science Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Faculty Of Agriculture Kagawa University

• KOTANI Hirokazu

  Kazusa DNA Res. Inst.

• Tajima S

  Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Takane Kenichi

  Institute of Agrobiological Resources

• Kouchi Hiroshi

  Institute of Agrobiological Resources

• Tajima Shigeyuki

  Fac. Agr. Kagawa University

• Tajima Shigeyuki

  Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University

• Guo Chen-xiang

  Dept. Life Sci. Fac. Agr. Kagawa Univ.

• Takane Kenichi

  Dept. Life Science Kagawa University

• Nomura Mika

  Faculty Of Agriculture Kagawa University Kagawa

• Guo Chen-xiang

  Fac. Of Agri. Kagawa Univ.

• Takane Ken-ichi

  Dept. Bioresource Sience Kagawa Univ.

• Kouchi Hiroshi

  Natl. Inst. Agrobiol. Resources.

• Kouchi Hiroshi

  Department Of Plant Sciences National Institute Of Agrobiological Sciences

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