Isolation and Characterization of a cDNA That Encodes a Novel Proteinase Inhibitor I from a Tobacco Genetic Tumor

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概要

• 論文の詳細を見る

• We have isolated a cDNA clone, designated GTI, by screening a tobacco genetic tumor cDNA library with a tumor-specific "subtracted" cDNA probe. The cDNA contained the entire coding sequence for a 94-amino-acid polypeptide that exhibited significant homology to members of the proteinase inhibitor I family from tomato and potato. The predicted protein has a pre-sequence of 22 amino acids but lacks a pro-sequence, unlike genes for proteinase inhibitor I isolated to date. Furthermore, the protein encoded by GTI cDNA has a novel reactive site, having glutamine as the P_1 reactive residue. These results suggest that the GTI protein is a novel member of the proteinase inhibitor I family. The mRNA for GTI accumulated at a high level but only transiently after the wounding of tobacco plants. Thus, it appears that the GTI protein has a function that is related to the protection of tissues against damage due to wounding.

• 日本植物生理学会の論文

著者

• Kouchi Hiroshi

  National Institute Of Agrobiological Sciences

• Kouchi Hiroshi

  National Institute Of Agrobiological Resources

• Kouchi Hiroshi

  Natio. Insti. Agrobiol. Res. Tsukuba Naist

• SYONO Kunihiko

  Department of Pure and Applied Sciences, The University of Tokyo

• Ichikawa Takanari

  Department Of Pure And Applied Sciences University Of Tokyo

• Ichikawa Takanari

  Department Of Pure And Applied Science University Of Tokyo

• Syono K

  Univ. Tokyo Tokyo Jpn

• Syono Kunihiko

  Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address

• FUJITA Tomomichi

  Department of Pure and Applied Sciences, University of Tokyo

• Ichikawa T

  Department Of Pure And Applied Sciences University Of Tokyo

• Fujita Tomomichi

  Department Of Biological Sciences Faculty Of Science Hokkaido University

• Fujita T

  Department Of Pure And Applied Sciences University Of Tokyo

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