Function of Ngrol Genes in the Evolution of Nicotiana glauca : Conservation of the Function of NgORF13 and NgORF14 after Ancient Infection by an Agrobacterium rhizogenes-Like Ancestor
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概要
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Ngrol genes are thought to have resulted from horizontal gene transfer from an Agrobacterium rhizogenes-like ancestor early in the evolution of the genus Nicotiana. Four Ngrol genes (NgrolB, NgrolC, NgORF13 and NgORF14) have been found in the genome of N.glauca, but their functions are not yet known. We have investigated the properties of Ngrol genes and shown that some of them are able to function in tobacco plants. Transgenic analysis revealed that NgORF13 promotes RirolB-mediated adventitious root induction on tobacco leaf segments. NgORF14 also promoted the RirolB-mediated root induction, but the intensity of this promoting effect was weak. These promoting functions of NgORF13 and NgORF14 have much the same efficiency as those of the corresponding genes of A.rhizogenes, RiORF13 and RiORF14, respectively. Overexpression of NgORF13, under control of the cauliflower mosaic virus 35S promoter (P_<35S>), provoked morphological abnormalities in transgenic tobacco plants. Transgenic plants that harbored the P_<35S>-NgORF13 had rounded leaves and stout flowers resulting from suppression of the longitudinal growth of leaf and floral leaves such as sepals, petals, stamens and carpels. These results suggest that NgORF13 and NgORF14 in the genome of N.glauca have conserved functional sequences since their original integration event by an A.rhizogenes-like ancestor.
- 日本植物生理学会の論文
著者
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SYONO Kunihiko
Department of Pure and Applied Sciences, The University of Tokyo
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Syono Kunihiko
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address
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Aoki S
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address
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Aoki Seishiro
Department Of Biology Graduated School Of Arts And Sciences The University Of Tokyo
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