Isolation of Rhizobium leguminosarum biovar viciae Variants with Indole-3-acetamide Hydrolase Activity

スポンサーリンク

概要

• 論文の詳細を見る

• The IAA biosynthetic pathway from tryptophan to IAA via IAM (IAM pathway) was investigated in Rhizobium spp. (fast-growing rhizobium). Southern hybridization with the bam gene, a structural gene for IAM hydrolase (the enzyme that converts IAM to IAA) cloned from Bradyrhizobium japonicum J 1063, indicated that homologous sequences exist among wild-type Rhizobium spp. However the IAM pathway has not been detected biochemically in free-living bacteria. When 5-methyltryptophan-resistant strains were screened for Rhizobium leguminosarum biovar viciae K5 which has DNA sequences with high homology to the bam gene, spontaneous mutants showing IAM hydrolase activity were isolated. The results suggest the possibility that the activity of IAM hydrolase is suppressed in free-living state in Rhizobium leguminosarum biovar viciae K5. In addition we detected the peak at the same t_R Of IAM by HPLC analysis using two columns when a large amount of L-tryptophan was added to the suspension of 5-methyltryptophan-resistant variants. Whether or not tryptophan-2-monooxygenase activity, however, actually works in Rhizobium cells remained to be solved.

• 日本植物生理学会の論文

著者

• SEKINE Masami

  Department of Bioproduction Science, Faculty of Bioresources and Environmental Sciences, Ishikawa Pr

• Syono Kunihiko

  Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address

• Sekine Masami

  Department Of Fermentation Technology Faculty Of Engineering Osaka University

• Kawaguchi M

  Univ. Tokyo Tokyo Jpn

• Kawaguchi Masayoshi

  Department Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Syono Kunihiko

  Department Of Pure And Applied Sciences College Of Arts And Sciences The University Of Tokyo

• Sekine Masami

  Department Of Bioproduction Science Ishikawa Prefectural University

関連論文

• Lotus japonicus Infested with Herbivorous Mites Emits Volatile Compounds That Attract Predatory Mites
• Lotus japonicus CLAVATA2 negatively regulates nodulation
• Occurrence of Brassinolide and Castasterone in Crown Gall Cells of Catharanthus roseus(Biological Chemistry)
• Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle
• Different Expression Patterns of the Promoters of the NgrolB and NgrolC Genes during the Development of Tobacco Genetic Tumors
• The Regulatory Functions of the rolB and rolC Genes of Agrobacterium rhizogenes are Conserved in the Homologous Genes (Ngrol) of Nicotiana glauca in Tobacco Genetic Tumors : GENE STRUCTURE AND EXPRESSION : GENE STRUCTURE AND EXPRESSION
• Nodule Organogenesis in Lotus japonicus
• Physiological studies of secondary metabolites in Curcuma species : production in vitro and effects on other organisms
• Detection of the IAA Biosynthetic Pathway from Tryptophan via Indole-3-Acetamide in Bradyrhizobium spp
• Function of Ngrol Genes in the Evolution of Nicotiana glauca : Conservation of the Function of NgORF13 and NgORF14 after Ancient Infection by an Agrobacterium rhizogenes-Like Ancestor
• Endogenous Levels of Gibberellins, IAA and Cytokinins in Catharanthus Crown Gall Tissues of Different Tumor Types
• Morphological Observations and Qualitative and Quantitative Studies of Auxins after Induction of Tobacco Genetic Tumor
• Endogenous Levels of Gibberellins, IAA and Cytokinins in Tobacco Crown Gall Tissues of Different Morphologies
• The Excessive Production of Indole-3-Acetic Acid and Its Significance in Studies of the Biosynthesis of This Regulator of Plant Growth and Development
• Unusual Branching in the Seedlings of Lotus japonicus-Gibberellins Reveal the Nitrogen-sensitive Cell Divisions within the Pericycle on Roots
• Efficient Conversion of L-Tryptophan to Indole-3-Acetic Acid and/or Tryptophol by Some Species of Rhizoctonia
• Isolation and Characterization of a cDNA That Encodes a Novel Proteinase Inhibitor I from a Tobacco Genetic Tumor
• Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange
• Changes in Morphology, Levels of Endogenous IAA and Protein Composition in Relation to the Development of Tobacco Genetic Tumor Induced in the Dark
• The Presence of an Enzyme that Converts Indole-3-acetamide into IAA in Wild and Cultivated Rice
• Induction of Cell Division Synchrony and Variation of Cytokinin Contents through the Cell Cycle in Tobacco Cultued Cells
• Tumorization-Redifferentiation System of Tobacco Genetic Tumor
• The Identification of Indole-3-Acetic Acid and Indole-3-Acetamide in the Hypocotyls of Japanese Cherry
• Two Ineffective-Nodulating Mutants of Lotus japonicus-Different Phenotypes Caused by the Blockage of Endocytotic Bacterial Release and Nodule Maturation-
• Requirement for Mesorhizobium loti Ornithine Transcarbamoylase for Successful Symbiosis with Lotus japonicus as Revealed by an Unexpected Long-Range Genome Deletion
• How Many Peas in a Pod? Legume Genes Responsible for Mutualistic Symbioses Underground
• Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Translation is Regulated in a Small Subunit-Independent Manner in the Expanded Leaves of Tobacco
• The Novel Symbiotic Phenotype of Enhanced-Nodulating Mutant of Lotus japonicus : astray Mutant is an Early Nodulating Mutant with Wider Nodulation Zone
• Shoot-applied MeJA Suppresses Root Nodulation in Lotus japonicus
• Nod Factor/Nitrate-Induced CLE Genes that Drive HAR1-Mediated Systemic Regulation of Nodulation
• Expression and Promoter Activity of Genes for Isozymes of Horseradish Peroxidase : GENE STRUCTURE AND EXPRESSION
• Synergistic Function of rolB, rolC, ORF13 and ORF14 of TL-DNA of Agrobacterium rhizogens in Hairy Root Induction in Nicotiana tabacum
• Photosynthesis-Dependent Volume Regulation in Guard Cell Protoplasts from Vicia faba L.
• Effect of CO_2 on Volume Change of Guard Cell Protoplast from Vicia faba L.
• A exopolysaccharide-defective mutant of Rhizobium loti eliciting abnormal nodulation
• Pollen Development and Tube Growth are Affected in the Symbiotic Mutant of Lotus japonicus, crinkle
• Rhizobium Attachment and Curling in Asparagus, Rice and Oat Plants
• Hair Curling Induced in Heterologous Legumes and Monocots by Flavonoid-Treated Rhizobia
• Morphological Effects of Sinefungin, an Inhibitor of S-Adenosylmethionine-Dependent Methyltransferases, on Anabaena sp. PCC 7120
• Genetic Analysis of the Effects of Polar Auxin Transport Inhibitors on Root Growth in Arabidopsis thaliana
• Tobacco Genetic Tumors
• Vapor Phase Epitaxial Growth of ZnTe Films Using Metallic Sources
• Growth-Stimulation of Tobacco Plant Introduced the Horseradish Peroxidase Gene prxCla
• Isolation of Rhizobium leguminosarum biovar viciae Variants with Indole-3-acetamide Hydrolase Activity
• Partial purification of an enzyme hydrolyzing indole-3-acetamide from rice cells
• Cultivation of Rice Protoplasts and Their Transformation Mediated by Agrobacterium Spheroplasts
• Physiological Role of Leghaemoglobin Heterogeneity in Pea Root Nodule Development
• Effects of Leghaemoglobin Components on Nitrogen Fixation and Oxygen Consumption
• Hormonal Control of Elongation of Tobacco Cells Derived from Protoplasts
• Correlation between induction of auxin-nonrequiring tobacco calluses and increase inhibitor (s) of IAA-destruction activity
• Current Development of Lotus japonicus Research
• Cryptogein-Induced Cell Cycle Arrest at G_2 Phase is Associated with Inhibition of Cyclin-Dependent Kinases, Suppression of Expression of Cell Cycle-Related Genes and Protein Degradation in Synchronized Tobacco BY-2 Cells
• SLEEPLESS, a gene conferring nyctinastic movement in legume
• Lotus japonicus 'Miyakojima' MG-20: An Early-Flowering Accession Suitable for Indoor Handling
• TOO MUCH LOVE, a Novel Kelch Repeat-Containing F-box Protein, Functions in the Long-Distance Regulation of the Legume-Rhizobium Symbiosis

もっと見る 閉じる

スポンサーリンク