Cultivation of Rice Protoplasts and Their Transformation Mediated by Agrobacterium Spheroplasts
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概要
- 論文の詳細を見る
Improvement of the cultivation of rice (Oryza sativa L.) protoplasts isolated from suspension cultures led to their division at a frequency of 5 to 10%. Rapidly growing colonies were obtained on a hormone-free medium when Agrobacterium tumefaciens sphero-plasts were introduced into the protoplasts by polyethylene glycol treatment. Opines corresponding to the strains of A. tumefaciens used for the spheroplast treatments were detected in some of these colonies at a frequency of about 10^<-4>. Using radioactive precursors, [^<14>C]-a-ketoglutaric acid and [3^H]-arginine, activities of nopaline synthase, a marker enzyme of nopaline-type crown gall. were also detected in some of these clones. These results show that the rice cells were transformed by Ti plasmid introduced by the spheroplast method.
- 日本植物生理学会の論文
著者
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Baba Akiko
Department Of Pure And Applied Sciences College Of Arts And Sciences University Of Tokyo
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Syono Kunihiko
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address
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Hasezawa Seiichiro
Department Of Pure And Applied Sciences College Of Arts And Sciences University Of Tokyo
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Hasezawa Seiichiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Syono Kunihiko
Department Of Pure And Applied Sciences College Of Arts And Sciences University Of Tokyo
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