The Presence of an Enzyme that Converts Indole-3-acetamide into IAA in Wild and Cultivated Rice
スポンサーリンク
概要
- 論文の詳細を見る
The enzymatic conversion of indole-3-acetamide (IAM) to IAA, which is the second step in the IAM pathway (tryptophan → IAM → IAA) was investigated in calluses derived from various dicotyledonous and monocotyledonous plants. A simple method, using analysis by HPLC to measure the conversion of naphthaleneacetamide (NAM) to naphthaleneacetic acid (NAA) was employed for the detection of IAM hydrolase activity. Among calluses from 27 plants tested, only callus from a cultivated strain of rice (Oryza sativa C5924) had high conversion activity similar to that of crown gall cells, and very weak activity was found in calluses from lucern and orange. In addition to the presence of the conversion activity, we confirmed that radioactivity from 3^H-IAM was incorporated into IAA in a cell-free system from O. sativa C5924. An extract of roots of rice seedlings exhibited twice the activity of that in an extract of shoots. IAM hydrolase activity was observed in calluses from all lines of rice callus examined, irrespective of whether they were wild or cultivated lines, with the exception of O. grandiglumis W 1194 and O. branchyantha W656, while other species of Gramineae exhibited no activity. These results suggest the possibility that this enzyme may play a specific role in rice.
- 日本植物生理学会の論文
著者
-
SAKURAI Akira
The Institute of Physical and Chemical Research
-
KAWAGUCHI Masayoshi
Department of Life Science, Graduate School of Arts and Sciences, University of Tokyo
-
Sakurai A
The Institute Of Physical And Chemical Research (riken)
-
Sakurai Akira
The Inst. Of Phys. And Chem. Res.
-
Kobayashi Masatomo
The Institute of Physical and Chemical Research
-
SYONO Kunihiko
Department of Pure and Applied Sciences, The University of Tokyo
-
Kobayashi Masatomo
The Inst. Of Phys. And Chem. Res.
-
Kobayashi Masatomo
Plant Growth Regulation Laboratory The Institute Of Physical And Chemical Research (rlken)
-
Syono K
Univ. Tokyo Tokyo Jpn
-
Syono Kunihiko
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address
-
Katsumi M
The Inst. Of Phys. And Chem. Res.
-
Kawaguchi Masayoshi
Dept. Life Sciences Tokyo Univ.
-
Kawaguchi Masayoshi
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
-
Kobayashi Masaru
Plant Nutrition Laboratory Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto Univers
関連論文
- Isolation and Identification of (-)-Jasmonic Acid from Wild Rice, Oryza officinalis, as an Antifungal Substance(Organic Chemistry)
- Lotus japonicus Infested with Herbivorous Mites Emits Volatile Compounds That Attract Predatory Mites
- Structural Analyses of Carbon Chains in 5-Alk(en)ylresorcinols of Rye and Wheat Whole Flour by Tandem Mass Spectrometry
- Structures of Oryzalides A and B, and Oryzalic Acid A, a Group of Novel Antimicrobial Diterpenes, Isolated from Healthy Leaves of a Bacterial Leaf Blight-resistant Cultivar of Rice Plant(Organic Chemistry)
- Identification of the Female Sex Pheromone of the Cabbage Webworm
- Amino Acid Sequence of αk Substance, a Mating Pheromone of Saccharomyces kluyveri
- Chemical Structure of Mating Pheromone Peptides, α^ and α^ Pheromones, Produced by Mating Type α Cells of Saccharomyces kluyveri(Organic Chemistry)
- Partial Characterization of a 17 kDa Acidic Protein, EFP, Induced by Thiocarbamate in the Early Flowering Phase in Asparagus Seedlings
- CHARACTARIZATION OF EFP (EARLY FLOWERING PROTEIN) IN ASPARAGUS
- 41 Thiocarbamate-Induced Early Flowering Protein is Expressed in Male Asparagus Flower
- Cloning of the cDNA encoding a novel PR-like protein induced by thiocarbamate during early flowering phase in Asparagus seedlings.
- C-55 Characterization of a novel PR-like protein induced by thiocarbamate during early flowering phase in Asparagus seedlings
- cDNA CLONING OF ENT-KAURENE SYNTHETASEB FROM THE IMMATURE SEEDS OF PUMPKIN
- Effects of a Plant-Growth Regulator, Prohexadione-Calcium (BX-112), on the Endogenous Levels of Gibberellins in Rice
- Effects of a Plant-Growth Regulator, Prohexadione, on the Biosynthesis of Gibberellins in Cell-Free Systems Derived from Immature Seeds
- Effects of a New Plant Growth Regulator Prohexadione Calcium (BX-112) on Shoot Elongation Caused by Exogenously Applied Gibberellins in Rice (Oryza sativa L.) Seedlings
- Lotus japonicus CLAVATA2 negatively regulates nodulation
- Studies on a Quantitative Analysis of Oryzalides and Oryzalic Acids in Rice Plants by GC-SIM
- Structures of Oryzalic Acid B and Three Related Compounds, a Group of Novel Antibacterial Diterpenes, Isolated from Leaves of a Bacterial Leaf Blight-Resistent Cultivar of Rice
- Novel C_-Kaurane Type of Diterpene (Oryzalide A), a New Antimicrobial Compound Isolated from Healthy Leaves of a Bacterial Leaf Blight-resistant Cultivar of Rice Plant(Organic Chemistry)
- Structures of Two Novel Pyriculol-related Compounds and Identification of Naturally Produced Epipyriculol from Pyricularia oryzae(Organic Chemistry)
- Effect of Ethylene and Gibberellin on the Elongation of Rice Seedlings(Oryza sativa L. )
- Effect of Ethylene and Gibberellin on theElongation of Rice Seedlings (Oryza sativa L.)
- Biological Activities of Biosynthetically-related Congeners of Brassinolide
- Identification of a New Brassinosteroid, Cathasterone, in Cultured Cells of Catharanthus roseus as a Biosynthetic Precursor of Teasterone
- Possible Involvement of 3-Dehydroteasterone in the Conversion of Teasterone to Typhasterol in Cultured Cells of Catharanthus roseus
- Identification of Brassinosteroid-like Active Substances in Plant-cell Cultures
- Occurrence of Brassinolide and Castasterone in Crown Gall Cells of Catharanthus roseus(Biological Chemistry)
- Occurrence of Castasterone, Brassinolide and Methyl 4-Chloroindole-3-acetate in Immature Vicia faba Seeds(Organic Chemistry)
- Changes in Endogenous Gibberellin Contents during Growth of Cultued Tobacco Cells
- Gibberellin Production in Cultured Cells of Nicotiana tabacum
- Effects of Fluorogibberellins on Plant Growth and Gibberellin 3β-Hydroxylases
- Effects of Ethylene and Gibberellins on the Elongation of Rice Seedlings (Oryza sativa L.)
- Inhibition of ent-Kaurene Synthase by Quaternary Ammonium Growth Retardants
- Effects of 3-Methylgibberellin Analogs on Gibberellin 3β-Hydroxylases and Plant Growth
- ENDOGENOUS GIBBERELLIN LEVELS DURING GERMINATION OF BARLEY SEEDS
- Fluctuation of Endogenous Gibberellin and Abscisic Acid Levels in Germinating Seeds of Barley
- Nodule Organogenesis in Lotus japonicus
- Specific Accumulation of Antifungal 5-Alk(en)ylresorchinol Homologs in Etiolated Rice Seedlings
- Structure of a New Antifungal C_-Hydroxyfatty Acid Isolated from Leaves of Wild Rice (Oryza officinalis)
- Toxic Activity of Mimics of the Host-Specific Pathotoxin PM-Toxin on Corn Carrying Texas Male-sterile Cytoplasm(Organic Chemistry)
- Detection of the IAA Biosynthetic Pathway from Tryptophan via Indole-3-Acetamide in Bradyrhizobium spp
- Endogenous Levels of Gibberellins, IAA and Cytokinins in Catharanthus Crown Gall Tissues of Different Tumor Types
- Morphological Observations and Qualitative and Quantitative Studies of Auxins after Induction of Tobacco Genetic Tumor
- Endogenous Levels of Gibberellins, IAA and Cytokinins in Tobacco Crown Gall Tissues of Different Morphologies
- The Excessive Production of Indole-3-Acetic Acid and Its Significance in Studies of the Biosynthesis of This Regulator of Plant Growth and Development
- Brassinolide Is Biosynthesized from Castasterone in Catharanthus roseus Crown Gall Cells(Organic Chemistry)
- Unusual Branching in the Seedlings of Lotus japonicus-Gibberellins Reveal the Nitrogen-sensitive Cell Divisions within the Pericycle on Roots
- PCP Award PRESENCE OF A PATHWAY FOR THE BIOSYNTHESIS OF AUXIN VIA INDOLE-3-ACETAMIDE IN TRIFOLIATA ORANGE.
- Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange
- The Presence of an Enzyme that Converts Indole-3-acetamide into IAA in Wild and Cultivated Rice
- ANALYSIS OF ENDOGENOUS GIBBERELLINS ANDα-AMYLASE ACTIVITY IN GERMINATING RICE SEEDS
- Identification of Brassinolide, Castasterone, Typhasterol, and Teasterone from the Pollen of Lilium elegans
- DISTRIBUTION OF GA, IAA AND ABA IN WEEPING AND UPRIGHT TYPES OF JAPANESE CHERRY, Prunus spachiana
- ENDOGENOUS GIBBERELLIN IN NORMAL AND WEEPING CHERRY
- Analysis of Endogenous Gibberellins and Abscisic Acid in Vegetative Shoots of Normal and Weeping Japanese Cherry (Prunus spachiana)
- ent-Kaurene Synthesis and Endogenous Levels of Gibberellins in a Shoot Forming Tobacco Crown Gall Tissue
- Effects of the new plant growth retardants of quaternary ammonium iodides on gibberellin biosynthesis in Gibberella fujikuroi
- Sites of Inhibition by a Plant-Growth Regulator, 4'-Chloro-2'-(a-hydroxybenzyl)-isonicotinanilide (Inabenfide), and its Related Compounds in the Biosynthesis of Gibberellins
- The Identification of Indole-3-Acetic Acid and Indole-3-Acetamide in the Hypocotyls of Japanese Cherry
- Two Ineffective-Nodulating Mutants of Lotus japonicus-Different Phenotypes Caused by the Blockage of Endocytotic Bacterial Release and Nodule Maturation-
- Biosynthesis of Brassinosteroids in Seedlings of Catharanthus roseus, Nicotiana tabacum, and Oryza sativa
- Requirement for Mesorhizobium loti Ornithine Transcarbamoylase for Successful Symbiosis with Lotus japonicus as Revealed by an Unexpected Long-Range Genome Deletion
- How Many Peas in a Pod? Legume Genes Responsible for Mutualistic Symbioses Underground
- The Novel Symbiotic Phenotype of Enhanced-Nodulating Mutant of Lotus japonicus : astray Mutant is an Early Nodulating Mutant with Wider Nodulation Zone
- Metabolism of Gibberellins in Cell-Free Extracts of Anthers from Normal and Dwarf Rice
- Quantitative Analysis of Endogenous Gibberellins in Normal and Dwarf Cultivars of Rice
- Fluctuation of the Endogenous IAA Level in Rice during Its Life Cycle(Organic Chemistry)
- Effects of Deoxygibberellin C (DGO and 16-Deoxo-DGC on Gibberellin 3β-Hydroxylase and Plant Growth
- Conversion of GA_5 to GA_6 and GA_3 in Cell-free Systems from Phaseolus vulgaris and Oryza sativa(Organic Chemistry)
- Partial Purification and Characterization of Gibberellin 3β-hydroxylase from Immature Seeds of Phaseolus vulgaris L
- Metabolism of [^C]GA_ in a Cell-Free System from Developing Seeds of Phaseolus vulgaris L.
- Isolation of a Gibberellin Biosynthesis Inhibitor from Testas of Phaseolus vulgaris L.(Organic Chemistry)
- Shoot-applied MeJA Suppresses Root Nodulation in Lotus japonicus
- Nod Factor/Nitrate-Induced CLE Genes that Drive HAR1-Mediated Systemic Regulation of Nodulation
- C1-65 Influence of development stages of Asparagus seed embryo on flower induction
- Identification of Castasterone, 6-Deoxocastasterone, Typhasterol and 6-Deoxotyphasterol from the Shoots of Arabidopsis thaliana
- Induction of sexual agglutinability of a mating-type cells as the primary action of the peptidyl sex factor from α mating-type cells in Saccharomyces cerevisiae
- Identification of Teasterone and Phytosterols in the Lipid Fraction from Seeds of Cannabis sativa L.
- Gibberellin A_ Is Converted to Gibberellin A_ in Cultured Cells of Nicotiana tabacum and Catharanthus roseus
- Gibberellin Transport from the Cotyledon to Plumule in the Flowering of Pharbitis nil
- 39 Involvement of Endogenous IAA and ABA in the Flowering of Pharbitis nil
- Effect of Uniconazole and Gibberellin on the Flowering of Pharbitis nil
- Involvement of GA transport from cotyledon to plumules in the flowering of Pharbitis nil
- C-53 EFFECTS OF UNICONAZOLE AND GIBBERELLINS ON THE FLOWERING OF PHARBITIS NIL
- Distribution of Endogenous Gibberellins in Vegetative Shoots of Rice : GROWTH AND DEVELOPMENT
- Identification and Quantification of Abscisic Acid, Indole-3-acetic Acid and Gibberellins in Phloem Exudates of Pharbitis nil
- Gibberellins and Flowering of Pharbitis nil
- Fluctuation of Endogenous Gibberellin Levels in the Early Development of Rice
- A2-10 ANALYSIS OF PLANT HORMONES IN PHLOEM EXUDATESFROM COTYLEDONS OF PHARBITIS NIL
- Effects of a Plant Growth Regulator, Prohexadione Calcium (BX-112), on the Elongation of Rice Shoots Caused by Exogenouslyl Applied Gibberellins and Helminthosporol, Part II
- Isolation and Identification of _L-Pipecolic Acid and Nicotinamide as Flower-Inducing Substances in Lemna
- Flowering and Endogenous Levels of Plant Hormones in Lremna Species
- Isolation and Identification of Lutein from Lemna paucicostata 381 as an Inhibitor of Benzoic Acid-induced Flowering in Lemna paucicostata 151(Biological Chemistry)
- Studies on Biosynthesis of Brassinosteroids
- A exopolysaccharide-defective mutant of Rhizobium loti eliciting abnormal nodulation
- Pollen Development and Tube Growth are Affected in the Symbiotic Mutant of Lotus japonicus, crinkle
- Isolation and Identification of Xanthoxin from Marine Plants(Organic Chemistry)
- Physiological detection of a binding substance for the agglutinability-inducing pheromone, α substance-I in Saccharomyces cerevisiae
- Structure/Activity Relationships of Grandinol: a Germination Inhibitor in Eucalyptus