Chemical Structure of Mating Pheromone Peptides, α^<sk1> and α^<sk3> Pheromones, Produced by Mating Type α Cells of Saccharomyces kluyveri(Organic Chemistry)

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Three peptides, α^<sk1>, α^<sk2> and α^<sk3> pheromones, have been isolated as α-mating pheromones of Saccharomyces kluyveri, the primary structure of the main active component, α^<sk2> pheromone, having already been determined. The unknown N-terminus of α^<sk1> pheromone was elucidated to be 1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (β-CAR) by mass and NMR spectrometric analyses. Synthetic β-CAR-His-Trp-OH was identical with N-terminal tripeptide fragment obtained from α^<sk1> pheromone, and the primary structure of α^<sk1> pheromone was determined as β-CAR-His-Trp-Leu-Ser-Phe-Ser-Lys-Gly-Glu-Pro-Met(O)-Tyr-OH. The amino acid sequence of α^<sk3> pheromone was determined as H-Trp-His-Trp-Leu-Ser-Phe-Ser-Lys-Gly-Glu-Pro-Met-OH by comparing the enzymatic fragments with those of α^<sk2> pheromone.
社団法人日本農芸化学会の論文
1986-05-23