An Ergosterol Peroxide, a Natural Product That Selectively Enhances the Inhibitory Effect of Linoleic Acid on DNA Polymerase β

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概要

• 論文の詳細を見る

• As described previously (Mizushina Y., Tanaka N., Yagi H., Kurosawa T., Onoue M., Seto H., Horie T., Aoyagi N., Yamaoka M., Matsukage A., Yoshida S., and Sakaguchi K., Biochim. Biophys. Acta, 1308,256-262,1996), linoleic acid (LA) inhibits the activities of mammalian DNA polymerases. We found a natural product from a basidiomycete, Ganoderma lucidum, that enhances this effect of LA in a special manner. The structure was identified to be an ergosterol peroxide, 5,8-epidioxy-5α, 8α-ergosta-6,22E-dien-3β-ol by spectroscopic analyses. The ergosterol peroxide (EPO) itself scarcely inhibited the activities of calf thymus DNA polymerase α(pol. α) or rat DNA polymerase β(pol. β). However, when EPO at 0.25 mM was present, 10 μM or less of LA almost completely inhibited the pol. β activity, while almost complete inhibition by LA itself was acheived at 80 μM or higher. Interestingly, under the same conditions, EPO did not affect the LA-effect on pol. α. The action mode of the EPO was discussed.

• 公益社団法人日本薬学会の論文
• 1998-05-15

著者

• SAKAGUCHI Kengo

  Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Sci

• SUGAWARA Fumio

  Department of Applied Biological Science, Tokyo University of Science

• KOSHINO Hiroyuki

  The Institute of Physical and Chemical Research (RIKEN)

• Yoshida S

  Plant Science Center Riken Institute

• Yoshida S

  Cancer Immunotherapy Center Nagoya Kyoritu Hospital

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Yoshida Shonen

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• ESUMI Yasuaki

  The Institute of Physical and Chemical Research, RIKEN

• Mizushina Yoshiyuki

  Department of Nutritional Science, Kobe-Gakuin University

• UZAWA Jun

  The Institute of Physical and Chemical Research

• Togashi Hideaki

  Department Of Applied Biological Science Science University Of Tokyo

• WATANABE Itiro

  Department of Applied Biological Science, Science University of Tokyo

• HANASHIMA Linda

  Department of Applied Biological Science, Science University of Tokyo

• TAKEMURA Masaharu

  Laboratory of Cancer Cell Biology, Research Institute for Disease Mechanism and Control, Nagoya Univ

• OHTA Keisuke

  Department of Applied Biological Science, Science University of Tokyo

• MATSUKAGE Akio

  Laboratory of Cell Biology, Aichi Cancer Center Research Institute

• Suzuki Y

  Department Of Applied Biological Chemistry The University Of Tokyo

• Matsukage A

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Matsukage Akio

  Department Of Chemical And Biological Sciences Japan Women's University

• Matsukage Akio

  Laboratory Of Cell Biology

• Watanabe Itiro

  Department Of Applied Biological Science Science University Of Tokyo

• Hanashima Linda

  Department Of Applied Biological Science Science University Of Tokyo

• TAKEMURA Masaharu

  Life Science Research Center, Mie University

• Koshino H

  Riken (the Inst. Physical And Chemical Res.) Saitama Jpn

• Koshino Hiroyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University:(present Address) Ri

• Koshino H

  Molecular Characterization Team Advanced D&s Center Riken

• Koshino Hiroyuki

  Molecular Characterization Characterization Center Riken(the Institute Of Physical And Chemical Rese

• Yoshida Shigeki

  Inst. Applied Biochem. Tsukuba Univ.

• Takemura Masaharu

  Life Science Research Center Mie University

• Sakagichi Ken-ichi

  Institute For Protein Research Osaka University

• Sugawara F

  Dep. Of Applied Biological Sci. Tokyo Univ. Of Sci.

• Uzawa J

  The Institute Of Physical And Chemical Research (riken)

• Uzawa Jun

  The Institute Of Physical And Chemical Research (riken)

• Sugawara Fumio

  Dep. Of Applied Biological Sci. Tokyo Univ. Of Sci.

• Sugawara Fumio

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Ohta Keisuke

  Department Of Anatomy & Histology Kurume University School Of Medicine

• Esumi Y

  Riken (the Institute Of Physical And Chemical Research)

• Esumi Yasuaki

  The Institute Of Physical And Chemical Research (riken)

• Esumi Yasuaki

  The Institute Of Physical And Chemical Research

• Takemura M

  Life Science Research Center Mie University

• Mizushima Y

  Laboratory Of Food And Nutritional Sciences Department Of Nutritional Science Kobe-gakuin University

• Mizushina Y

  Kobe‐gakuin Univ. Kobe Jpn

• Ohta Keisuke

  Department Of Applied Biological Science Frontier Research Center For Genomic Drug Discovery Tokyo U

• Sakaguchi Kengo

  Department Of Applied Biological Science Tokyo University Of Science

• Sakaguchi Kengo

  Department Of Applied Biological Science Faculty Of Science And Technology Tokyo University Of Scien

• Sakaguchi Kengo

  Department Of Applied Biological Science Faculty Of Science And Technology Science University Of Tok

• Mizushina Yoshiyuki

  Department Of Nutritional Science Kobe Gakuin University

• Koshino Hiroyuki

  Advanced Science Institute Riken

• Koshino Hiroyuki

  The Institute Of Physical & Chemical Research (riken)

• Sugawara Fumio

  Dep. Of Applied Biological Sci. Fac. Of Sci. And Technol. Tokyo Univ. Of Sci.

• Takemura Masaharu

  Laboratory Of Biology Education Graduate School Of Mathematics And Science Education Tokyo Universit

• Mizushina Yoshiyuki

  Department of Applied Biological Science, Science University of Tokyo

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