Direct Detection for β-Xylanase on Isoelectric Focusing Gels by Using 2,3,5-Triphenyltetrazolium Chloride

スポンサーリンク

概要

• 論文の詳細を見る

• Specific detection of β-xylanase activity on isoelectric focusing gels was done by using 2,3,5-triphenyltetrazolium chloride (TTC). Reducing sugars released from water-soluble xylan with β-xylanase reacted with TTC, and then xylanase activities appeared as pink-red bands on the gel. The sensitivity of detection for β-xylanase by this procedure was higher than that of protein detection with Coomassie Brilliant Blue R-250.

• 社団法人日本農芸化学会の論文
• 1995-03-23

著者

• Kusakabe Isao

  lnstitute of Applied Biochemistry, University of Tsukuba

• Kaneko Satoshi

  Food Biotechnology Div. National Food Res. Inst.

• Yoshida S

  Plant Science Center Riken Institute

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Kaneko Satoshi

  National Food Res. Inst.

• Kaneko S

  Food Biotechnology Division National Food Research Institute

• Yoshida Shigeki

  lnstitute of Applied Biochemistry, University of Tsukuba

• Kaneko Satoshi

  lnstitute of Applied Biochemistry, University of Tsukuba

• Matsuo Noriki

  lnstitute of Applied Biochemistry, University of Tsukuba

• Yoshida Shigeki

  Inst. Applied Biochem. Tsukuba Univ.

• Kusakabe I

  Univ. Tsukuba Tsukuba Jpn

• Matsuo N

  Department Of Applied Chemistry Faculty Of Engineering Nagasaki University

関連論文

• Chemical Structure of Xylan in Cotton-seed Cake(Biological Chemistry)
• Preparation of Glucuronoxylo-oligosaccharides from an Acid Hydrolysate of Corn-hulls(Biological Chemistry)
• Action of Soymilk-clotting Enzyme from Bacillus sp. K-295G-7 on the Acidic Subunit of Soybean 11S Globulin(Biological Chemistry)
• Application of Soymilk-whey to Production of Beverage Containing α-D-Galactosyl Oligosaccharides(Food & Nutrition)
• Cloning and Sequence Analysis of cDNA for Irpex lacteus Aspartic Proteinase(Biological Chemistry)
• Substrate Specificity of Soymilk-clotting Enzymes I and II from Bacillus sp. K-295G-7 toward Peptide Substrates (Biological Chemistry)
• Purification and Characterization of Extracellular Poly(β-D-1, 4-mannuronide) Lyase from Dendryphiella salina IFO 32139
• Preparation of Dye-labeled Alginate for the Assay of Alginate Lyase
• Preparation of Two Series of Oligo-guluronic Acids from Sodium Alginate by Acid Hydrolysis and Enzymatic Degradation
• Purification and Characterization of Extracellular Alginate Lyase from Enterobacter cloacae M-1
• Structure of Rice-straw Arabinoglucuronoxylan and Specificity of Streptomyces Xylanase toward the Xylan(Biological Chemistry)
• Molecular cloning of cDNAs encoding two glycoside hydrolase family 7 cellobiohydrolases from the basidiomycete Flammulina velutipes
• Crystallographic Snapshots of an Entire Reaction Cycle for a Retaining Xylanase from Streptomyces olivaceoviridis E-86
• The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea
• Cross talk between auxin and other hormones in the early process of transdifferentiation into tracheary elements
• Effect of Diesel Exhaust on Development of Fetal Reproductive Function in ICR Female Mice
• Maternal Exposure to Diesel Exhaust Decreases Expression of Steroidogenic Factor-1 and Mullerian Inhibiting Substance in the Murine Fetus
• Effect of Dibromochloropropane (DBCP) on the Hormone Receptors of the Male Rat Reproductive System
• The Effects of Diesel Exhaust Particles (DEPs) on a Murine Testis (Proceedings of the 22nd Symposium on Toxicology and Environmental Health)
• Cadmium Concentration in Grains of Japanese Wheat Cultivars : Genotypic Difference and Relationship with Agronomic Characteristics(Genetic Resources Evaluation)
• Uzu Mutation in Barley (Hordeum vulgare L.) Reduces the Leaf Unrolling Response to Brassinolide(Organic Chemistry)
• Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene
• Identification of a Peptide Mimic of Bioactive Gibberellins with Affinity to GA 2-Oxidase
• Sequential Regulation of Gibberellin, Brassinosteroid, and Jasmonic Acid Biosynthesis Occurs in Rice Coleoptiles to Control the Transcript Levels of Anti-Microbial Thionin Genes
• ISOLATION OF BRASSINOSTEROID DEFICIENCY-INSENSITIVE MUTANTS OF ARABIDOPSIS
• Analysis of the gene expression in response to brassinosteroids by using Gene Chip
• Light regulation of brassinosteroid-biosynthetic genes in Arabidopsis thaliana
• Cloning of Brassinosteroid-6-Oxidase from Arabidopsis thaliana
• Regulatory Mechanism of Photomorphogenesis by Pytohormones -An Analysis with Brassinosteroid-Biosynthesis-Specific Inhibitor, Brassinazole (Brz)
• EFFECTS OF SPECIFIC BRASSIONSTEROID (BR) BIOSYNTHESIS INHIBITOR (BRASSINAZOLE : Brz) ON PLANTS
• BRASSINOSTEROID BIOSYNTHESIS INHIBITOR : STRUCTURE, ACTIVITY AND TARGET SITES
• Crystallization and Preliminary X-ray Crystallographic Study of Streptomyces olivaceoviridis E-86 β-Xylanase
• Screening for Phenoloxidases from Edible Mushrooms(Biochemistry & Molecular Biology)
• Purification and Characterization of an α-L-Arabinofuranosidase from suspension cultured Non-embryo genic carrot (Daucus carota. L)
• PCR Cloning and Expression of the F/10 Family Xylanase Gene from Streptomyces olivaceoviridis E-86
• Substrate Specificity of the α-L-Arabinofuranosidase from Trichoderma reesei
• Substrate Specificites of α-L-Arabinofuranosidases Produced by Two Species of Aspergillus niger
• Purification and Characterization of α-Glucuronidase from Snail Acetone Powder
• Chloroisonicotinamide Derivative Induces a Broad Range of Disease Resistance in Rice and Tobacco
• Thiadiazole carboxylic acid moiety of tiadinil, SV-03, induces systemic acquired resistance in tobacco without salicylic acid accumulation
• Identification of Three Clones Which Commonly Interact with the Kinase Domains of Highly Homologous Two Receptor-Like Kinases, RLK902 and RKL1
• Molecular Characterization of Two Highly Homologous Receptor-like Kinase Genes, RLK902 and RKL1, in Arabidopsis thaliana
• Synthesis of a Novel Class of Polyhydroxyalkanoates in Arabidopsis Peroxisomes, and Their Use in Monitoring Short-Chain-Length Intermediates of β-Oxidation
• Application of the Random Arbitrary Primed Polymerase Chain Reaction Differential Display Method to Isolate Genes of Cholesterol Metabolism-related Proteins from Rat Liver
• Syntheses of 4-Methylumbelliferyl-β-D-Xylobioside and 5-Bromo-3-Indolyl-β-D-Xylobioside for Sensitive Detection of Xylanase Activity on Agar Plates
• Purification and Characterization of a Family G/11 β-Xylanase from Streptomyces olivaceoviridis E-86
• Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
• Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
• Characterization of a Thermostable Endo-β-1,4-D-galactanase from the Hyperthermophile Thermotoga maritima
• Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
• Expression of Streptomyces coelicolor α-Galactosidase Gene in Escherichia coli and Characterization
• An Ergosterol Peroxide, a Natural Product That Selectively Enhances the Inhibitory Effect of Linoleic Acid on DNA Polymerase β
• Screening of Inhibitor of Eukaryotic DNA Polymerases Produced by Microorganisms
• Purification, Characterization, and cDNA Cloning of a Novel α-Galactosidase from Mortierella vinacea
• Purification and Some Properties of α-Galactosidase from Penicillium purpurogenum
• Galactosyldiacylglycerol, a Mammalian DNA Polymerase α-Specific Inhibitor from a Sea Alga, Petalonia bingbamiae
• The Cyanogenic Glucoside, Prunasin (D-Mandelonitrile-β-D-Glucoside), Is a Novel Inhibitor of DNA Polymerase β
• Action of a New Mammalian DNA Polymerase Inhibitor, Sulfoquinovosyldiacylglycerol
• Phytotoxins from the Septoria spp. Plant Pathogenic Fungus on Leafy Spurge
• Sulfoquinovosyldiacylglycerol, KM043,a New Potent Inhibitor of Eukaryotic DNA Polymerases and HIV-Reverse Transcriptase Type 1 from a Marine Red Alga, Gigartina tenella
• Phytoactive Eremophilanes Produced by the Weed Pathogen Drechslera gigantea
• Effects of Partial Suppression of Ribosomal Protein S6 on Organ Formation in Arabidopsis thaliana(Biochemistry & Molecular Biology)
• Purification and Characterization of Recombinant Mortierella vinacea α-Galactosidases I and II Expressed in Saccharomyces cerevisiae
• Direct Detection for β-Xylanase on Isoelectric Focusing Gels by Using 2,3,5-Triphenyltetrazolium Chloride
• Detection of α-L-Arabinofuranosidase Activity in Isoelectric Focused Gels using 6-Bromo-2-naphthyl-α-L-arabinofuranoside
• Resistance to Protoporphyrinogen Oxidase-inhibiting Compound S23142 from Overproduction of Mitochondrial Protoporphyrinogen Oxidase by Gene Amplification in Photomixotrophic Tobacco Cells(Organic Chemistry)
• Intracellular targeting and distribution of protoporphyrinogen oxidase-II involved in chlorophyll and heme biosynthesis
• Purification and Properties of Protoporphyrinogen Oxidase from Spinach Chloroplasts
• Molecular characterization and subcellular localization of chloroplastic protoporphyrinogen oxidase in spinach
• Superiority of Dietary Safflower Oil over Olive Oil in Lowering Serum Cholesterol and Increasing Hepatic mRNAs for the LDL Receptor and Cholesterol 7α-Hydroxylase in Exogenously Hypercholesterolemic (ExHC) Rats
• Classification of Brassinosteroid-regulated Genes Based on Expression Profiles in bril and in Response to a Protein Kinase Inhibitor, Staurosporin
• Brassinosteroid Selectively Regulates PIN Gene Expression in Arabidopsis
• Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
• AtFLA11, a Fasciclin-Like Arabinogalactan-Protein, Specifically Localized in Screlenchyma Cells
• ENZYMIC FERULOYLATION OF ARABINOXYLAN OLIGOSACCHARIDE BY AN ENZYME EXTRACTED FROM SUSPENSION CULTURED RICE CELLS
• Structure of xylan from culms of bamboo grass (Sasa senanensis Rehd.)
• Effects of Unsaturated Uronic Acid Residues at Non-reducing End on Bond Cleavage Frequency of Poly(1, 4-α-L-guluronide) Lyase from Enterobacter cloacae M-1
• Substrate Specificities of α-Galactosidases from Yeasts
• The Carboxyl Terminal Sequence of Nucleolar Protein B23.1 Is important in Its DNA Polymerase α-Stimulatory Activity
• Nucleolar Protein B23.1 Binds to Retinoblastoma Protein and Synergistically Stimulates DNA Polymerase α Activity^1
• Genomic Organization and Alternative Splicing of Human PACE4 (SPC4), Kexin-Like Processing Endoprotease
• Human Subtilisin-Like Proprotein Convertase, PACE4 (SPC4) Gene Expression Is Highly Regulated through E-Box Elements in HepG2 and GH4C1 Cells
• Characterization of an Exo-β-1,3-D-galactanase from Streptomyces avermitilis NBRC14893 Acting on Arabinogalactan-Proteins
• Expression of Rice (Oryza sativa L. var. Nipponbare) α-Galactosidase Genes in Escherichia coli and Characterization
• Structure of Hardwood Xylan and Specificity of Streptomyces β-Xylanase toward the Xylan
• Characterization of Glycoside Hydrolase Family 6 Enzymes from Coprinopsis cinerea
• Development of a Gene Transfer System for the Mycelia of Flammulina velutipes Fv-1 Strain
• Properties of Ethanol Fermentation by Flammulina velutipes
• Use of Whole Crop Sorghums as a Raw Material in Consolidated Bioprocessing Bioethanol Production Using Flammulina velutipes
• Analysis of Calcium and Magnesium Ions in Wheat Flour by Capillary Zone Electrophoresis
• Molecular Cloning and Expression in Pichia pastoris of a Irpex lacteus Exo-β-(1→3)-galactanase Gene
• The Tetramer Structure of the Glycoside Hydrolase Family 27 α-Galactosidase I from Umbelopsis vinacea
• Hyper-Phosphorylated Retinoblastoma Protein Suppresses Telomere Elongation
• Improvement of the Transformation Efficiency of Flammulina velutipes Fv-1 Using the Glyceraldehyde-3-phosphate Dehydrogenase Gene Promoter
• Cloning and Transcript Analysis of Multiple Genes Encoding the Glycoside Hydrolase Family 6 Enzyme from Coprinopsis cinerea
• Effects of Cellulose Crystallinity, Hemicellulose, and Lignin on the Enzymatic Hydrolysis of Miscanthus sinensis to Monosaccharides
• Long Terminal Repeat of the Mushroom Retrotransposon Functions as a Dual Terminator to Transcription
• Distribution of Hydrophobin 1 Gene Transcript in Developing Fruiting Bodies of Lentinula edodes(Biochemistry & Molecular Biology)
• Extracellular Carbohydrate Esterase from the Basidiomycete Coprinopsis cinerea Released Ferulic and Acetic Acids from Xylan
• Effect of Lime Pretreatment of Brown Midrib Sorghums

もっと見る 閉じる

スポンサーリンク