Purification and Characterization of Extracellular Poly(β-D-1, 4-mannuronide) Lyase from Dendryphiella salina IFO 32139
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概要
- 論文の詳細を見る
An extracellular endo poly(β-D-1, 4-mannuronide) lyase of Dendryphiella salina IF 32139 was purified to homogeneity by Q Sepharose FF and Sephacryl S-200 HR column chromatographies. The purified enzyme had a molecular weight of 35,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and an isoelectric point of 3.65 by isoelectric focusing. The optimum pH and temperature for enzyme activity were pH 5.0 and 45℃, respectively. The enzyme was stable from pH 4 to 10 and at temperature below 40℃. Some divalent cations, Ca^<2+>, Mn^<2+>, and Zn^<2+>, increased the enzyme activity. Hg^<2+> and NBS strongly inhibited the activity. This enzyme susceptibly degraded poly-M, produced a wide range of 4,5-unsaturated oligomannuronic acids, and further degraded these unsaturated oligomannuronic acids to produce the unsaturated monomer and dimer as final products.
- 社団法人日本農芸化学会の論文
- 1997-04-23
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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KOBAYASHI Hideyuki
National Astronomical Observatory of Japan
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Kobayashi H
National Food Research Institute
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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YOSHIDA Shigeki
Institute of Applied Biochemistry, University of Tsukuba
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MURATA Katsumi
Research Development Section, Kibun Food Chemifa Co., Ltd.
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SHIMOKAWA Tomoko
Institute of Applied Biochemistry, University of Tsukuba
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TAKEUCHI Toshio
Research and Development Department, Kibun Food Chemifa Co., Ltd.
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Yoshida S
Plant Science Center Riken Institute
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Yoshida S
Cancer Immunotherapy Center Nagoya Kyoritu Hospital
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Yoshida S
Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer
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Kiuchi Hiroshi
Research Development Section Kibun Food Chemifa Co. Ltd.
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Murakami K
Univ. Tsukuba Ibaraki Jpn
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Murata K
Kobe Oji Zoo Kobe Jpn
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Takeuchi Toshio
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Yoshida Shigeki
Inst. Applied Biochem. Tsukuba Univ.
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SHIMOKAWA Tomoko
Forestry and Forest Products Res. Inst.
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Yoshida Shigeki
Institute Of Applied Biochemistry University Of Tsukuba
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MURATA Katsumi
Research and Development Department, Kibun Food Chemifa Co., Ltd.
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