Purification and Characterization of Endo Poly (α-L-Guluronate) Lyase in the Enzyme System from Flavobacterium multivolum
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概要
- 論文の詳細を見る
An alginate lyase was purified from a crude enzyme of Flavobacterium multivolum K-11 by successive column chromatographies, such as cation exchange, chromatofocusing, and gel filtration. The enzyme thus obtained migrated as a single band on SDS-PAGE. The relative molecular mass of the enzyme was 43-kDa by SDS-PAGE and 41-kDa by HPLC gel filtration chromatography. The isoelectric point of the enzyme was 8.7. The enzyme exhibited maximum activity at pH 8.0 and 40°C, and was stable in the pH range of 6.0 to 9.0 and at temperatures up to 30°C. The enzyme activity was remarkably inhibited by chemical compounds such as EDTA, PCMB, MIA, TNBS, and N-bromosuccinimide. The enzyme was specific for poly-guluronate and produced several kinds of oligomers. Thus, the results suggested that the enzyme was classified as endo poly (α-L-guluronate) lyase (EC 4.2.2.11).
- 社団法人 日本食品科学工学会の論文
著者
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Murakami K
Research And Development Department Kibun Food Chemifa Co. Ltd.
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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NIBU Yutaka
Institute of Applied Biochemistry, University of Tsukuba
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TAKEUCHI Toshio
Development Section Kamogawa Plant, Kibun Food Chemifa Co., Ltd.
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MURATA Katsumi
Saitama Plant, Kibun Food Chemifa Co., Ltd.
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Murata K
Kobe Oji Zoo Kobe Jpn
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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