Structures of Glucomanno-oligosaccharides from the Hydrolytic Products of Konjac Glucomannan Produced by a β-Mannanase from Streptomyces sp.
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概要
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The hydrolysis of konjac glucomannan by a purified extracellular mannanase from Streptomyces sp. produced four kinds of oligosaccharides containing both glucose and mannose residues. These glucomanno-oligosaccharides were identified as O-β-D-glucopyranosyl-(1→4)-Dmannopyranose (epicellobiose, G1M1-I), 0-β-D-glucopyranosyl-(1→4)-O-β-D-mannopyranosyl- (1→4)-D-mannopyranose (G2M1-I), O-β-glucopyranosyl-(1 →4)-O-β-D-glucopyranosyl-(1→4)-D-mannopyranose (G2M1-I) and O-β-D-glucopyranosyl-(1 →4)-O-β-D-glucopyranosyl-(1→4)-O-β-D-mannopyranosyl-(1→4)-D-mannopyranose (G2M2-I). On the basis of the structure of the above oligosaccharides, the mode of action of the mannanase on the glucomannan is discussed.
著者
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Kusama Satoru
Institute Of Applied Biochemistry University Of Tsukuba
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Sakurai Yoshio
Department Of Anesthesiology Saitama Medical Center Saitama Medical University
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Murakami Kazuo
Institute Of Applied Biochemistry University Of Tsukuba
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MAEKAWA Akio
Department of Agricultural Chemistry, Tokyo University of Agriculture
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SUZUKI Takao
Department of Agricultural Chemistry, Tokyo University of Agriculture
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TAKAHASHI Rihei
Department of Agricultural Chemistry, Tokyo University of Agriculture
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