Transglucosylation into Stevioside by the Enzyme System from Streptomyces sp.(Biological Chemistry)
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概要
- 論文の詳細を見る
The enzyme system from Streptomyces sp. W19-1 formed several kinds of transfer products (TPs) when incubated in the presence of both stevioside (ST) and curdlan. Three of the major TPs (A, B, and C) were separated and purified using HP-20 column chromatography, gel filtration on TOYOPEARL HW-40F, and preparative high-performance liquid chromatography. The structures of the three were identified by chemical and enzymatic methods; A is 13-O-β-sophorosyl-19-O-β-laminaribiosyl steviol, B is 13-O-β-3^2-β-glucosylsophorosyl-19-O-β-glucosyl steviol, and C is 13-O-β-sophorosyl-19-O-β-laminaritriosyl steviol. The three were obtained for the first time in a pure state.
- 社団法人日本農芸化学会の論文
- 1986-10-23
著者
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Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba
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Murakami Kazuo
Institute of Applied Biochemistry, University of Tsukuba
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KUSAMA Satoru
Institute of Applied Biochemistry, University of Tsukuba
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Nakamura Y
Kyoto Univ. Kyoto Jpn
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EDA Shigeru
Central Research Institute, The Japan Tobacco and Salt Public Corporation
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Kusakabe I
Univ. Tsukuba Tsukuba Jpn
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Eda Shigeru
Central Research Institute Nippon Tabacco Co. Ltd.
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Kusakabe Isao
Institute Of Applied Biochemistry University Of Tsukuba
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Kusama Satoru
Institute Of Applied Biochemistry University Of Tsukuba
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NAKAMURA Yasuyuki
Institute of Applied Biochemistry, University of Tsukuba
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Murakami K
Tokai University School Of Medicine
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Murakami Kazuo
Institute Of Applied Biochemistry University Of Tsukuba
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Nakamura Yasushi
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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